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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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相关实验视频

Updated: Jun 22, 2025

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

使用FLAIR2检测长读数中的单 haplotype 特定的转录变异.

Alison D Tang¹, Colette Felton¹, Eva Hrabeta-Robinson¹

¹Department of Biomolecular Engineering, University of California, Santa Cruz, USA.

|July 3, 2024

概括

长读测序能够同时分析RNA变异和拼接变化. 这种方法澄清了腺-氨酸编辑酶ADAR在肺癌进展中的作用.

关键词:

在A-to-I编辑中.阿达尔 (ADAR) 是一个叫做ADAR的词.一个闪耀的火焰长时间读取的RNA-seq.

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

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Last Updated: Jun 22, 2025

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

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Published on: June 23, 2012

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Published on: August 4, 2016

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

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Published on: December 9, 2016

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

RNA测序 (RNA-seq) 揭示了与疾病相关的RNA处理异常.
异常拼接和RNA单核酸变体 (SNVs) 影响转录稳定性,局部化和功能.
ADAR酶的上调与肺腺癌的侵袭性和拼接调节有关.

研究的目的:

为了克服短读RNA-seq在询问RNA变体和拼接同时进行的局限性.
为了阐明变体对单个分子水平上的拼接变化的 cis 效应,使用长读序列.
通过识别氨酸异形协会,澄清ADAR在瘤发生中的作用.

主要方法:

利用长读序列来获得全长的转录序列.
开发了一个增强FLAIR的计算工作流程,以集成RNA变异调用与异型.
由肺腺癌细胞生成的纳米孔测序数据,有或没有ADAR敲击.

主要成果:

从长时间读取数据中成功集成RNA变异调用与相关异型.
确定了与ADAR在瘤发生中的作用相关的关键氨酸异型关联.
演示了开发工作流程在分析复杂RNA变异方面的能力.

结论:

长读测序为RNA变异和拼接模式关系提供了宝贵的见解.
开发的计算工作流提高了RNA变异的分析.
这种方法有助于理解RNA编辑和拼接在疾病中的功能重要性.