相关实验视频

Updated: Jul 15, 2026

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Published on: May 14, 2015

使用实验设计方法解决生物反应器hiPSC总稳定性,维护和扩展挑战

Haneen Yehya^1,2, Sofija Raudins¹, Roshan Padmanabhan¹

¹Trailhead Biosystems, 23215 Commerce Park, Beachwood, OH, 44122, USA.

Stem cell research & therapy

|July 3, 2024

概括

在悬浮培养中优化介质添加剂可增强诱导多能干细胞 (iPSC) 的扩张和稳定性. 这种方法控制了总体大小,提高了再生疗法的可扩展性.

科学领域:

细胞生物学细胞生物学
生物技术是生物技术.
再生医学是一种再生医学.

背景情况:

来自干细胞的疗法为再生医学提供了前景.
静态细胞培养方法在可扩展性方面存在局限性.
悬浮培养使大规模的细胞生产成为可能,但在应力和总体稳定性方面存在挑战.

研究的目的:

在悬浮培养中优化介质添加剂,用于大规模诱导多能干细胞 (iPSC) 扩张.
为了增强细胞生长,保持多能性,并确保生物反应器培养期间的总稳定性.
为媒体和生物反应器优化开发预测性数学模型.

主要方法:

在垂直轮生物反应堆中采用了实验设计 (DOE) 方法.
评估了多功能介质添加剂,包括肝素盐 (HS),聚乙烯糖醇 (PEG),聚 (乙烯醇) (PVA),Pluronic F68和硫酸盐 (DS).
创建并调整数学模型以预测基于添加输入的细胞生长,多能性和总稳定性.

主要成果:

优化的介质组合 (PA,PVA,PEG与E8) 与单独E8相比,减少了40%的iPSC扩张翻倍时间.
将1%的PEG添加到E8介质中对于保持多能性至关重要.
氨酸和PEG的相互作用最大限度地减少了聚合物融合,增强了hiPSC的扩张和维护,这对于大规模种植至关重要.
在经过验证的优化条件下,维持了高多能标志物表达 (>90%) 和在降低的生物反应器速度 (40 RPM) 时的翻倍时间为 1-1,4 天.

结论:

在悬浮培养物中控制聚合物大小,从而同时控制iPSC状态.
开发的方法解决了媒介优化复杂性和生物反应器扩展对干细胞治疗的挑战.
这种方法促进了iPSCs在治疗应用中的高效和稳定的大规模扩展.

关键词:

总体大小总体大小总体大小总体稳定性总体稳定性聚合物聚合物聚合物生物反应器是一种生物反应器.文化媒体文化美国能源部 (DoE)人类诱导的多能干细胞干细胞.多能状态多能状态.核武器的扩散悬浮文化是一种悬浮文化.这就是 iPSC 的意义.

更多相关视频

Designing a Bioreactor to Improve Data Acquisition and Model Throughput of Engineered Cardiac Tissues

Designing a Bioreactor to Improve Data Acquisition and Model Throughput of Engineered Cardiac Tissues

Published on: June 2, 2023

Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor

Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension Bioreactor

Published on: March 17, 2023

相关概念视频

Bioreactor Design and Operational System

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...

Bioreactor Controls-I

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...

Bioreactor Controls-II

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

Designing Growth Media for Bioreactors

Designing Growth Media for Bioreactors

Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...

Scale-Up Processes

Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...

Upstream Processing

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...