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在层群中重型寄生性伤寒炎的形态分子鉴定:组织反应和细胞介导反应

Mohamed A El-Saied¹, Marwa M Attia², Marwa A Ibrahim³

¹Department of Pathology, Faculty of Veterinary Medicine, Cairo University, PO Box 12211, Giza, Egypt. mohamed.saied@vet.cu.edu.eg.

Acta veterinaria Scandinavica

|July 3, 2024

概括

分子方法准确地识别了层中的Heterakis gallinarum,揭示了明显的免疫反应和亡. 这项研究突出了诊断家禽寄生虫并了解其影响的新方法.

科学领域:

兽医寄生虫学兽医寄生虫学
分子诊断学分子诊断学
禽类的健康禽类的健康

背景情况:

Heterakis gallinarum是一种常见的家禽虫寄生虫,造成经济损失.
目前的诊断方法,如便卵数,是不可靠的,导致诊断不足.
缺乏分子鉴定阻碍了胃肠道线虫的快速诊断和控制.

研究的目的:

用形态学,超结构学和分子技术来描述埃及层中的Heterakis gallinarum.
为了研究感染后结组织中的组织病理和免疫反应.
建立精确的Heterakis诊断的分子方法,并了解宿主-寄生虫相互作用.

主要方法:

收集和检查受影响和未受影响的母的样.
使用光和扫描电子显微镜进行形态和超结构分析.
通过线粒体细胞氧化酶1 (COX1) 基因测序进行分子鉴定.
对炎症标记物 (IL-2,IFN-γ,TLR-4,IL-1β,IL-10) 和亡 (cas-3) 的基因表达的分析.

主要成果:

形态和COX1基因测序证实了Heterakis gallinarum的存在.
观察到促炎性细胞因子 (IL-2,IFN-γ,TLR-4,IL-1β) 的显著上调和IL-10的下调.
在受感染的部组织中检测到的亡活性 (cas-3).

结论:

分子方法,特别是COX1测序,为家禽中Heterakis感染提供了可靠的诊断.
这项研究是第一个报告了层层的特定组织免疫反应的研究,包括炎症标记物变化和亡.
研究结果提供了对宿主免疫反应的见解,以及对Heterakis感染的潜在治疗点.

关键词:

希特拉基斯 (Heterakis) 曾经是一个鱼.基因组移动迁移基因组移动迁移免疫反应的免疫反应.一层的寄生虫的寄生虫分子诊断是一种分子诊断.寄生性伤寒炎是一种寄生性伤寒炎.

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