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在体外和体内有紧 σ70 形状的证据

Khalil Joron1, Joanna Zamel1, Nir Kalisman1,2

  • 1Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Faculty of Mathematics & Science, Edmond J. Safra Campus, Hebrew University of Jerusalem, Jerusalem 9190401, Israel.

iScience
|July 3, 2024
PubMed
概括

大肠杆菌中的Apo-sigma70 (σ70) 存在于活细胞中的紧形式,与其与RNA聚合酶 (RNAP) 结合时的扩展状态不同. 这种紧的结构可能表明一个转录不活跃的状态.

关键词:
分子生物学分子生物学

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In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.