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内生性Aspergillus fumigatiaffinis:新的帕克利塔克塞尔生产和优化见解

Marwa Obiedallah¹, Alaa A Yasien², Sabah S Mohamed²

¹Microbiology Lab., Botany and Microbiology Department, Faculty of Science, Sohag University, Sohag, 82524, Egypt. m.obiedallah@science.sohag.edu.eg.

Applied microbiology and biotechnology

|July 3, 2024

概括

内生真菌,特别是来自Artemisia judaica的Aspergillus fumigatiaffinis,可以产生帕克利塔塞尔 (PTX). 优化显著增加了PTX产量,为癌症化疗提供了一个可持续的,环保的替代方案.

科学领域:

菌类学菌类学是指菌类学.
生物技术是生物技术.
自然产品化学化学

背景情况:

帕克利塔塞尔 (PTX) 是一种来自植物的关键化疗药物.
内生真菌为PTX生产提供了可持续和替代的来源.
亚特米西亚朱达伊卡 (Artemisia judaica) 拥有多种多样的内源性真菌,有可能产生二次代谢物合成.

研究的目的:

识别和表征来自Artemisia judaica根的内菌,能够产生帕克利塔克塞尔 (PTX).
评估和优化从有前途的真菌分离物中获得PTX产量.
确认一种特定的内菌菌株生产PTX的新奇性.

主要方法:

从Artemisia judaica根中分离和识别了100种内菌菌株.
使用薄层染色学 (TLC),高性能液体染色学 (HPLC) 和气体染色学-质谱学 (GC-MS) 进行PTX生产的选.
使用实验的全因数设计 (DOE) 和响应表面方法 (RSM) 优化PTX生产.

主要成果:

Aspergillus fumigatiaffinis被确定为产生PTX的最高分离物 (26.373μg L-1).
分子鉴定证实了该菌株是PTX生产的新型菌株.
优化条件 (pH 8.0,150μg L-1 贝可酶,18天) 提高了PTX产量,使每克干重达到110.23μg L-1.

结论:

内生性Aspergillus fumigatiaffinis是帕克利塔克塞尔 (PTX) 的新型来源.
优化策略显著提高PTX产量,使其成为植物提取的可行替代品.
这项研究为高度PTX生产提供了一种可持续和环保的方法.

关键词:

艾斯伯吉勒斯烟类植物实验的设计实验的设计固体植物性固体植物性优化优化优化优化帕克利塔克塞尔 (Paclitaxel) 是一种药物.在Taxol®中,我们可以使用Taxol®.

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