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对Leminorella grimontii及其染色体类A GRIβ-乳酸酶的基因组洞察

Claudia Aldeia¹, Edgar I Campos-Madueno^1,2, Andrea Endimiani³

¹Institute for Infectious Diseases (IFIK), University of Bern, Friedbühlstrasse 25, Bern, CH-3001, Switzerland.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

|July 3, 2024

概括

从昆虫幼虫中分离出来的Leminorella grimontii细菌具有GRI-1β-乳糖酶的染色体基因,GRI-1β-乳糖酶是一种赋予抗生素耐药性的酶. 这一发现为L. grimontiitii提供了新的见解.

科学领域:

微生物学微生物学
基因组学就是基因组学.
抗微生物耐药性抗微生物耐药性

背景情况:

莱米诺雷拉 (Leminorella grimontii) 是一种细菌物种.
这项研究研究了L. grimontii.的抗生素耐药性机制.

研究的目的:

为了描述L. grimontii菌株LG-KP-E1-2-T0.0的抗生素耐药性表型.
为了识别和分析L. grimontii.中的β-乳糖酶基因.
提供L. grimontii.第一个完整的基因组序列.

主要方法:

来自Zophobas morio幼虫的L. grimontii菌株LG-KP-E1-2-T0的分离和培养.
对扩展光谱β-乳糖酶活性进行表型敏感性测试.
在L. grimontii的全基因组测序LG-KP-E1-2-T0.0.
对β-乳糖酶基因 (blaGRI-1) 的生物信息分析和与其他L. grimontii基因组的比较.

主要成果:

L. grimontii LG-KP-E1-2-T0 具有可诱导的扩展光谱β-乳糖酶活性.
全基因组测序确定了一个染色体的bla基因编码GRI-1类Aβ-乳酸酶.
GRI-1β-乳糖酶与RIC-1和OXY型β-乳糖酶具有显著的氨基酸相同性.
blaGRI-1基因在公开可用的L. grimontii基因组中得到保存.
菌株LG-KP-E1-2-T0在分析的L. grimontii菌株中是基因组上独特的.

结论:

这项研究展示了L. grimontii的第一个完整基因组.
一种新的染色体类Aβ-乳酸酶,GRI-1,已经在L. grimontii.中得到了特征.
这些发现为beta-lactamase含量和L. grimontii.的基因组多样性提供了新的见解.

关键词:

莱米诺雷拉 (Leminorella) 是一个小鸟.佐福巴斯 (Zophobas) 是一个恐惧主义者.这就是ESBL.在GRI-1的基础上,GRI-1 基因组基因组基因组基因组基因组序列化是指测序的使用.在β-lactamases中,

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