比较现场评估和转录组分析显示,染色体倍增增强了大米膜炎抗性
Sanglin Liu1,2,3, Jiahao Liu1,3, Wei Wang1,3
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources; Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Rice (New York, N.Y.)
|July 3, 2024
概括
自体四化水一般比双化水表现出更好的田间抗病 (由Rhizoctonia solani引起) 的抵抗力. 染色体倍增增强了耐药性,尽管结果因基因型和环境而异.
科学领域:
- 植物病理学 植物病理学
- 遗传学 遗传学 是一个
- 农业学是一种农业学.
背景情况:
- 由Rhizoctonia solani引起的米病是大米生产的重大威胁.
- 由染色体倍增衍生出的自动四倍化大米具有改善特征的潜力,但其对疾病的耐药性尚不清楚.
研究的目的:
- 为了评估自带类大米基因型对病的野外耐药性.
- 为了研究染色体倍增对膜炎耐药性的影响.
- 阐明在自体四类动物中增强抗性的基础分子机制.
主要方法:
- 在三种环境中对35种自带四倍体和双倍体大米基因型的现场评估 (2020-2021年).
- 在最佳启动阶段用Rhizoctonia solani进行注射.
- RNA测序 (RNA-seq) 用于分析自身四倍体和双倍体在注射后的基因表达差异.
主要成果:
- 与双胞胎相比,自带四胞胎通常表现出较低的阴囊炎病得分.
- 染色体倍增增强了45.71%的基因型的耐药性,而48.57%的基因型表现出不稳定的耐药性.
- RNA-seq揭示了更多的下调差异表达基因 (DEGs),包括与耐药性相关的基因,在24小时后的Autotetraploids中.
- 乌比金/特尔化和特尔化生物合成途径与性特异性耐药性有关.
结论:
- 染色体的翻倍通常会提高米对囊病的野外抵抗力,但这种影响是基因型和环境依赖的.
- 特定的基因和通路的下调,如乌比昆/特尔化和特尔化生物合成,可能有助于提高自四米的耐药性.
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