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Updated: Jun 22, 2025

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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超快速热激发非adiabatic激发状态的动力学在绿色光蛋白染色体模拟器中

Bittu Lama1, Manabendra Sarma1

  • 1Department of Chemistry, Indian Institute of Technology, Guwahati, Assam 781039, India.

The journal of physical chemistry. B
|July 3, 2024
PubMed
概括

薄弱的π-π堆叠在化二甲基化中阻碍了扭转旋转,局部化了热刺激子. 这影响了生物系统中的光动力学和绿色光蛋白衍生物.

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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