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周围淋巴细胞的变化与婴儿死性肠球炎的进展有关
Shaojing Wu1, Xue Ren1, Yuxiu Zhuang1
1Department of Pediatric Surgery, Children's Hospital of Fudan University, Shanghai Key Laboratory of Birth Defect, Shanghai, China.
The Journal of surgical research
|July 3, 2024
概括
死亡性肠球炎 (NEC) 中的免疫细胞变化与疾病的严重程度相关. 特定的B和T淋巴细胞子集,特别是抗体分泌细胞和效应记忆T细胞,可能表明NEC患者需要手术.
科学领域:
- 免疫学 免疫学 免疫学
- 新生儿医学 新生儿医学
- 儿科手术 儿科手术
背景情况:
- 死性肠球炎 (NEC) 是婴儿严重的胃肠道疾病.
- 免疫系统的失调与NEC的发展有关.
- 有限的研究存在于NEC的外周血液淋巴细胞子集变化,特别是关于疾病严重程度和手术指示.
研究的目的:
- 在患有NEC的婴儿中调查外围血液B和T淋巴细胞子集的变化.
- 为了将这些免疫细胞变化与NEC疾病严重程度 (贝尔阶段) 相关联.
- 评估特定的淋巴细胞子集作为生物标记物的潜力,以识别需要手术干预的NEC患者.
主要方法:
- 一项病例控制研究,涉及34名患有NEC的婴儿和33名年龄匹配的对照.
- 在NEC诊断后48小时内采集的外周血液样本.
- 用于分析B和T淋巴细胞子集的12色流细胞计.
- 细胞比率的计算和评估它们与疾病严重程度和手术需要的关系.
主要成果:
- 与对照组相比,NEC患者在B和T淋巴细胞亚组中表现出明显的变化,包括原始,记忆和激活B细胞和CD4+/CD8+T细胞的比例变化.
- 患有高级NEC (贝尔阶段III) 的患者表现出切换记忆B细胞,抗体分泌细胞和效应记忆T细胞的增加,以及天真T细胞的减少.
- 接收器操作特征 (ROC) 曲线分析表明,对抗体分泌细胞/B细胞和效应记忆T细胞/CD4+T细胞的比率具有很高的灵敏度和特异性,可预测需要手术.
结论:
- 在NEC患者中,淋巴细胞成熟与疾病严重程度有直接的相关性,特别是涉及CD4+T细胞.
- 抗体分泌细胞/B细胞和效应记忆T细胞/CD4+T细胞比率的升高是识别可能需要手术的NEC患者的有希望的指标.
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