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相关概念视频

Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

542
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
542
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

376
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
376
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

1.9K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

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Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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Updated: Jun 22, 2025

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
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Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

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在疏水性相互作用色谱中模拟多元件分离,采用改进的参数逐参数估计方法.

Yu-Xiang Yang1, Zhi-Yuan Lin2, Yu-Cheng Chen1

  • 1Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang Key Laboratory of Smart Biomaterials, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310058, China.

Journal of chromatography. A
|July 3, 2024
PubMed
概括
此摘要是机器生成的。

一种新的参数估计方法 (mPbP-HIC) 提高了对蛋白质混合物的疏水相互作用色谱 (HIC) 模型的准确性. 该SLA+IM战略增强曲线适配和预测能力,加速生物制药工艺开发.

关键词:
疏水性相互作用色谱学 疏水性相互作用色谱学机械模型是机械模型.莫勒鲁普是一个热热体.多组件系统多组件系统.参数估计的参数估计.

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Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
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相关实验视频

Last Updated: Jun 22, 2025

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

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Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
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科学领域:

  • 生物化学工程 生物化学工程
  • 染色学分离科学 染色学分离科学
  • 工艺系统工程 工艺系统工程

背景情况:

  • 机械模型对于色谱过程的开发和优化至关重要.
  • 疏水性相互作用色谱 (HIC) 机械模型缺乏有效的参数估计,特别是在多组件系统中.
  • 准确的参数估计对于可靠的HIC过程建模和扩展至关重要.

研究的目的:

  • 开发和验证用于多组件HIC系统的新型参数估计方法 (mPbP-HIC).
  • 提高HIC机械模型的准确性和适用性,特别是在高负载条件下.
  • 提高生物制药工艺开发的HIC模型的预测能力.

主要方法:

  • 推导出一个参数对参数 (mPbP-HIC) 方法来估计Mollerup等热量的六个参数用于HIC.
  • 使用线性回归 (LR) 和线性近似 (LA) 进行初始参数估计,然后采用反向方法 (IM).
  • 引入了初始q_max估计的简化线性近似 (SLA),结合IM (SLA+IM) 进行改进的校准.

主要成果:

  • mPbP-HIC方法准确地预测了10 g/L负载时的蛋白质化.
  • 最初的LA步骤显示,由于实验条件和q_max误差,在较高负载 (12-14g/L) 上的安装不满意.
  • 改进的SLA+IM策略显著提高了曲线适配,减少了估计错误,并将错误积累最小化.

结论:

  • 通过SLA+IM策略,改进的mPbP-HIC方法变得更加合理,适用于实际的蛋白质混合物分离.
  • 增强的HIC模型展示了出色的预测能力和合理的推断,以优化流程.
  • 这种方法加速了下游生物制药制造中的HIC工艺开发.