提取方法对不同pH值的法巴豆球蛋白分量的结合能力的影响
Tiffany Amat1, Ali Assifaoui1, Johann Buczkowski2
1Université Bourgogne Franche-Comté, Institut Agro, Université Bourgogne, INRAE, UMR PAM 1517, 21000 Dijon, France.
Food chemistry
|July 3, 2024
概括
根据提取方法,法巴豆蛋白提取物与的结合方式不同. 性提取-同电沉 (AE-IEP) 显示出较高的结合比连续提取 (SE) 由于植物酸含量.
科学领域:
- 食品科学 食品科学 食品科学
- 营养生物化学 营养生物化学
- 植物蛋白提取 植物蛋白提取
背景情况:
- 法巴豆成分提供有价值的蛋白质和,但含有植物酸 (PA),这可能会阻碍矿物质的生物可用性.
- 植物酸 (PA) 与矿物质和蛋白质形成复合物,可能减少营养吸收.
研究的目的:
- 研究两种提取方法,即性提取-同电沉 (AE-IEP) 和顺序提取 (SE),如何影响法巴豆球蛋白系统的 (Ca) 结合能力.
- 了解pH和植物酸在法巴豆提取物中的蛋白相互作用中的作用.
主要方法:
- 准备了法巴豆蛋白分散剂 (2.5% w/v).
- 添加了化 (CaCl2) 在pH值 (4.5,5.5,6.5,7.5) 的不同度下.
- 监测自由离子度以评估结合能力.
主要成果:
- 结合在全球蛋白同电点 (pH ~4-5) 附近是最小的.
- 在更高的pH值下,发生了显著的结合,导致不溶性复合物的形成和与植物酸相关的蛋白质沉.
- 与SE球蛋白相比,AE-IEP提取的球蛋白表现出优异的结合,与更高的PA和较低的初始Ca相关.
结论:
- 提取方法显著影响了法巴豆球蛋白的结合潜力.
- 植物酸在法巴豆蛋白系统中的复合和沉中起着至关重要的作用.
- AE-IEP是一种更有效的方法来增强法巴豆蛋白成分中的结合.
相关概念视频
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