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循环RNAcircSLC16A10通过通过miR-761-5p/MFN2轴改善线粒体功能来缓解糖尿病视网膜病变

Lu Lu¹, Yuan Ning¹, Feng Gu²

¹Department of Ophthalmology, The Fourth Affiliated Hospital of China Medical University, Eye Hospital of China Medical University, Key Lens Research Laboratory of Liaoning Province, Shenyang, China.

Cellular signalling

|July 3, 2024

概括

循环RNA SLC16A10 (circSLC16A10) 在糖尿病视网膜病变 (DR) 中是下调的. 过度表达circSLC16A10通过通过miR-761-5p/MFN2通路改善线粒体功能来保护DR.

科学领域:

眼科医生眼科眼科
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

糖尿病视网膜病变 (DR) 是糖尿病的一个微血管并发症.
循环RNAs (circRNAs) 参与了DR的发病,但 circSLC16A10的作用尚不清楚.

研究的目的:

为了研究circSLC16A10在糖尿病视网膜病变中的功能.
阐明circSLC16A10在DR中的作用背后的分子机制.

主要方法:

使用的细胞 (ARPE-19) 和DR的动物模型.
分析了circSLC16A10表达水平的公共数据库.
进行过度表达和敲击实验.
研究了circSLC16A10,miR-761-5p和MFN2.2.之间的相互作用.

主要成果:

在DR患者,高葡萄糖治疗细胞和糖尿病小鼠中,circSLC16A10表达减少.
在实验室中,circSLC16A10的过度表达减少了内 плазма网膜应激,细胞亡和线粒体功能障碍.
circSLC16A10 作为miR-761-5p的海绵,增加了MFN2的表达.
circSLC16A10在体内证明了对DR的保护作用.

结论:

circSLC16A10在糖尿病视网膜病变中起着保护作用.
circSLC16A10通过通过miR-761-5p/MFN2轴增强线粒体功能来改善DR进展.

关键词:

糖尿病视网膜病变 - 糖尿病视网膜病变细胞内膜网膜应激压力是什么线粒体功能线粒体功能电路SLC16A1010 电路SLC16A10 电路SLC16A10 电路在 miR-761-5p/MFN2 轴上

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