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1,25-二氧维生素D₃增加了低剂量的基于PMA的THP-1细胞中单细胞到巨细胞的分化

Bronwyn A Mol¹, Janet J Wasinda¹, Yi F Xu¹

¹School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, WITS, Johannesburg 2050, South Africa.

Journal of immunological methods

|July 3, 2024

概括

醇12-米里-13-酸盐 (PMA) 对于THP-1细胞分化成巨细胞至关重要. 将PMA与1,25-二氧维生素D3 (1,25D3) 结合起来,可以增强巨细胞特征和M1/M2两极化.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

THP-1细胞系是一种标准的体外模型,用于研究单细胞与巨细胞的分化.
现有的使用博12-米里-13-酸盐 (PMA) 或1,25-二氧维生素D3 (1,25D3) 的差异化方案显示出显著的变异性.
协议差异可以影响由此产生的巨细胞特征.

研究的目的:

评估三个不同的THP-1分化协议的有效性.
为了比较由PMA,1,25D3或两者的组合诱导的巨细胞分化.
评估差异化方法对形态学和巨细胞标记物表达的影响.

主要方法:

使用三个条件区分THP-1细胞:5nM PMA,10nM 1,25D3或PMA和1,25D3.3的组合.
差异化之后是休息期.
分析了形态变化,基因表达和细胞表面标记物表达 (CD11b,CD14).

主要成果:

所有协议都增加了CD11b和CD14的表达.
单独的1,25D3并没有诱导巨细胞形态或特征表达.
PMA是巨细胞特征所必需的,由1,25D3.3增强.
PMA和PMA/1,25D3协议支持M1和M2两极分化.
PMA和1,25D3的组合显示出最明显的极化标记基因表达.

结论:

在THP-1细胞中,PMA对于诱导巨类特征至关重要.
PMA和1,25D3的组合优化了THP-1对巨细胞的分化.
这种联合协议似乎有助于对特定的M1或M2极化状态的承诺.

关键词:

1,25-二氧维生素D ((3)) 的不同化的差异化巨细胞的两极分化巨细胞是一个巨细胞.单细胞 (Monocytes) 是一种单细胞.在PMA中,PMA是PMA.在RNA-seqqq.在 THP-1 细胞中.

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