基于核桃蛋白分离的乳液作为一个有前途的输送系统,增强了黄蛋白生物可访问性
Liang Liu1, Lin Shan Shi1, Ching Yuan Hu2
1Engineering Research Center for High-Valued Utilization of Fruit Resources in Western China, Ministry of Education; National Research & Development Center of Apple Processing Technology; College of Food Engineering and Nutritional Science, Shaanxi Normal University, 620 West Changan Avenue, Xian, Shaanxi 710119, PR China.
International journal of biological macromolecules
|July 3, 2024
概括
修改的核桃蛋白分离乳液有效地稳定了黄蛋白,提高了其传递和生物可访问性. 超声波修饰在食品系统中对黄蛋白保留和生物可用性产生了最佳结果.
科学领域:
- 食品科学与技术 食品科学与技术
- 营养生物化学 营养生物化学
- 生物材料工程 生物材料工程
背景情况:
- 作为一种重要颜料的氨酸在食品加工过程中稳定性不佳,限制了其应用.
- 核桃蛋白分离物 (WNPI) 具有作为生物活性化合物的载体的潜力.
- 基于乳液的输送系统 (EBDS) 对于封装和保护敏感分子至关重要.
研究的目的:
- 开发和评估改进的基于WNPI的EBDS,用于增强黄蛋白强化.
- 研究修饰治疗对黄蛋白封装,稳定性和输送的影响.
- 在模拟的胃肠道条件下,从基于WNPI的EBDS评估黄蛋白的生物可访问性.
主要方法:
- 建造了四个修改的基于WNPI的EBDS,用于蛋白封装.
- 对封装效率,储存稳定性和消化稳定性的评估.
- 在体外消化试验测试以确定黄蛋白保留和生物可访问性.
- 分子对接模拟以阐明WNPI-蛋白相互作用.
主要成果:
- 修改治疗显著提高了基于WNPI的EBDS中的黄蛋白封装效率.
- 与未经处理的系统相比,修改后的EBDS显示了增强的储存和消化稳定性.
- 超声波修饰导致最大的黄蛋白保留 (49.67%) 和生物可访问性 (40.49%).
- 分子对接证实了WNPI和黄蛋白之间通过键和疏水相互作用的强有力的结合亲和力.
结论:
- 基于WNPI修改的EBDS是稳定和提供食品中黄蛋白的有效系统.
- 超声波修饰提供了一种有希望的策略,以最大限度地提高黄蛋白保留和生物可用性.
- 这项研究扩大了WNPI的应用,并为用其他脂溶性生物活性物强化食品提供了框架.
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