通过抑制PAI1蛋白表达,SERPINE1AS2通过抑制PAI1蛋白表达来调节肌内脂肪生成
Dianqi Zhang1, Xinhao Ma1, Huaxuan Li1
1College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
International journal of biological macromolecules
|July 3, 2024
概括
这项研究确定了SERPINE1AS2,一种新的长非编码RNA,作为牛肌内脂肪生成的关键调节者. 它对等离子素激活剂抑制剂-1和信号通路的调节会影响脂肪组织的发育.
科学领域:
- 分子生物学分子生物学
- 动物科学动物科学
- 遗传学 遗传学 是一个
背景情况:
- 反意义长非编码RNAs (lncRNAs) 是生物过程的关键调节者,包括牲畜中的脂肪组织积累.
- 在脂肪生成中,lncRNAs的特定功能和分子机制在很大程度上仍然未被描述,这代表了重要的知识差距.
研究的目的:
- 在牛脂肪组织中识别和描述新的反意义长非编码RNA SERPINE1AS2.
- 阐明SERPINE1AS2在调节肌肉内脂肪细胞增殖和分化的作用和分子机制.
主要方法:
- 在牛组织和脂肪细胞分化过程中识别和表达SERPINE1AS2的分析.
- 功能性研究涉及SERPINE1AS2.2.的淘汰和过度表达.
- RNA测序 (RNA-seq) 用于识别差异表达的基因和丰富的信号通路.
- 调查SERPINE1AS2和等离子体激活剂抑制剂-1 (PAI1) 之间的相互作用.
主要成果:
- 在牛脂肪组织中,SERPINE1AS2的表达很高,并且在肌肉内脂肪细胞分化过程中其表达增加.
- Knockdown 的 SERPINE1AS2 抑制脂肪细胞的增殖和脂肪生成,而过度表达促进了这些过程.
- 降低SERPINE1AS2导致MAPK,Wnt和mTOR信号通路中的DEGs得到显著的丰富.
- SERPINE1AS2与PAI1相互作用,影响其表达;PAI1下调减轻了 SERPINE1AS2减少对脂肪生成的抑制作用.
结论:
- 通过调节PAI1表达,SERPINE1AS2在牛肌内脂肪生成中起着至关重要的作用.
- 通过与MAPK,Wnt和mTOR信号通路的相互作用,SERPINE1AS2调节脂肪生成.
- 赛尔平1AS2在肌肉内脂肪细胞脂肪生成中表现出复杂的调节机制.
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