在OPU系统中的缺陷会导致对Weizmannia coagulans的盐敏感性
Tao Kim1,2, Sojeong Heo1, Jong-Hoon Lee3
1Department of Food and Nutrition, Dongduk Women's University, Seoul 02748, Republic of Korea.
Journal of microbiology and biotechnology
|July 3, 2024
概括
威兹曼尼亚凝结体由于奥斯摩保护剂吸收 (Opu) 系统的缺陷而表现出盐敏感性. 从Bacillus velezensis引入特定的OPU系统显著提高了W.coagulans.中的盐耐受性.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 遗传学 遗传学 是一个
背景情况:
- 威兹曼尼亚凝结菌是一种有价值的起始培养物和益生菌,但其应用受到盐敏感性的限制.
- 细菌的盐分耐受性通常由透保护剂吸收 (Opu) 系统介导,这些系统进口兼容的溶液以减轻透应激.
研究的目的:
- 为了研究威兹曼尼亚凝血菌盐敏感性的遗传基础.
- 为了确定特定的奥斯摩保护剂吸收 (Opu) 系统,赋予盐分耐受性.
主要方法:
- 对韦兹曼尼亚凝结菌和耐盐性细菌物种 (B. licheniformis,B. siamensis,B. subtilis,B. velezensis) 的比较基因组分析.
- 来自W. coagulans中的B. velezensis的Opu系统基因 (opuA, opuB, opuD, opuE) 的放大和表达.
- 通过测量不同NaCl度的生长,对工程W.coagulans菌株的盐耐受性进行评估.
主要成果:
- 威兹曼尼亚凝血菌只具有一个Opu系统 (OpuC),与具有多个系统 (OpuA-E) 的细菌菌种不同.
- 在W.coagulans中表达B. velezensis opuA, opuD或 opuE,使其在10.5%的NaCl中增长,而野生类型的3.5%.
- 过度表达B. velezensis Opu系统也提高了B. subtilis的盐耐药性.
结论:
- 威兹曼尼亚凝血菌对盐的敏感性归因于其有限的奥斯莫保护剂吸收系统.
- 具有异质Opu系统的工程W.凝固是一种可行的策略,用于改善其对食品发酵和益生菌应用的盐耐受性.
更多相关视频
11:58The Nematode Caenorhabditis Elegans - A Versatile In Vivo Model to Study Host-microbe Interactions
Published on: October 18, 2017
9.3K
11:50Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
Published on: October 1, 2015
21.8K
相关概念视频
02:02Responses to Salt Stress
13.1K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.1K
01:26Factors Affecting the Risk of Infection
11.6K
The hosts' susceptibility to infection depends on several factors. The integrity of the skin and mucous membranes helps protect the body against microbial attacks. When the skin is altered, the chance of infection, limb loss, and even death increases.
The integrity and count of the white blood cells help the body resist pathogens and fight infection. When impaired, it reduces the body's resistance to pathogens. The acidic pH levels of the gastrointestinal, genitourinary tracts, and skin...
The integrity and count of the white blood cells help the body resist pathogens and fight infection. When impaired, it reduces the body's resistance to pathogens. The acidic pH levels of the gastrointestinal, genitourinary tracts, and skin...
11.6K