在杂交猪中异位基因特异性调节变异的多原子特征
Jianping Quan1,2,3,4, Ming Yang5,6, Xingwang Wang1,7
1State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
Nature communications
|July 3, 2024
概括
这项研究利用多组数据绘制了猪组织和发育阶段的基因调节图. 它揭示了组织类型显著影响基因调节,而不是发育时间.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 动物科学动物科学
背景情况:
- 了解 cis 调节变异对于描述基因功能至关重要.
- 混合映射为识别cis调节基因提供了一种有效的策略.
- 像杜罗克和卢拉伊这样的猪品种表现出显著的表型分歧.
研究的目的:
- 为了全面描述多种猪组织和发育阶段的调控变异.
- 为了生成迄今为止最大的猪多原子数据集之一.
- 为了研究DNA甲基化,染色质可访问性和调控变异中的基因表达的相互作用.
主要方法:
- 使用了杜罗克和卢拉伊猪品种之间的相互交叉.
- 产生了广泛的多原子数据:全基因组测序,全基因组双硫酸盐测序,ATAC-Seq和RNA-Seq.
- 开发了一种基于阅读计数的新方法,用于评估等位基特异性甲基化,染色质可访问性和RNA表达.
主要成果:
- 与发育阶段相比,组织特异性强烈影响了DNA甲基化,染色质可访问性和基因表达.
- 鉴定了573个基因,表现出异位基因特异性表达,受原生基因和异位基因型的影响.
- 已证明,等位基特异性表达在很大程度上归因于等位基特异性甲基化和/或染色质可访问性.
结论:
- 提供了对不同组织和发育阶段的猪监管变化的详细描述.
- 强调组织特异性监管机制的重要作用.
- 建立了理解猪中cis调节元素驱动的基因型-表型关系的基础.
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