通过组合工程策略在Yarrowia lipolytica中有效合成烯生产
Young-Kyoung Park1,2, Lara Sellés Vidal1, David Bell3
1Department of Bioengineering and Centre for Synthetic Biology, Imperial College London, London, SW72AZ, UK.
Biotechnology for biofuels and bioproducts
|July 3, 2024
概括
这项研究设计了酵母,以可持续地生产利蒙. 将合成途径向过氧体显著增强了利蒙的生产,提供了一种新的微生物生产方法.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
背景情况:
- 烯是一种有价值的化合物,具有多种工业应用.
- 传统的植物提取在满足工业对利蒙的需求方面面临挑战.
- 微生物细胞工厂为烯合成提供了一个可持续的替代方案.
研究的目的:
- 为了对油性酵母 *Yarrowia lipolytica *进行工程设计,以生产D-和L-烯.
- 通过基因表达和细分来优化利蒙合成途径.
- 为了在Y. lipolytica*中实现高度的利蒙生产.
主要方法:
- 通过表达关键基因的工程 *Yarrowia lipolytica *: 利蒙烯合成酶 (LS),HMG-CoA减少酶 (HMG),杰拉尼尔二酸盐合成酶 (ERG20) 和内里尔二酸盐合成酶 (NDPS1).
- 评估个体和融合基因表达策略,以获得最佳的利蒙产量.
- 研究过氧体细分对利蒙生产的影响.
- 通过MVA路径向优化发酵条件和前体供应.
主要成果:
- 确定了一种最佳的菌株,将HMGR和融合蛋白ERG20-LS结合起来,用于烯生产.
- 获得的初始标位为29.4 mg/L L-烯和24.8 mg/L D-烯.
- 通过对合成基因的氧体向,证明了改善了利蒙的产生.
- 通过向MVA通路到氧体,将D-烯的产量增加到47.8 mg/L.
- 通过发酵优化达到69.3 mg/L的最终D-烯位.
结论:
- 成功设计了Y. lipolytica,用于高效的利蒙生物生产.
- 氧体对利蒙合成途径的向增强了酵母中的生产.
- 这项研究确立了Y. lipolytica作为烯生物合成的有希望的平台.
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