使用益生菌进行非酒精性脂肪胰腺管理的实验研究
Marwa Matboli1, Hiba S Al-Amodi2, Shaimaa Hamady3
1Medical biochemistry and molecular biology department, Faculty of medicine, Ain Shams University, Cairo, 11566, Egypt. DrMarwa_Matboly@med.asu.edu.eg.
工程化益生菌ZBiotic通过降低miR-5192和相关基因的调节,有效地治疗大鼠的非酒精性脂肪胰腺炎 (NAFP). 这种治疗逆转了纤维化,炎症和代谢功能障碍,显示了NAFP管理的希望.
科学领域:
- 代谢障碍 代谢障碍 代谢障碍
- 胃肠病学 胃肠病学
- 微生物学 微生物学
背景情况:
- 非酒精性脂肪胰腺炎 (NAFP) 是一种代谢障碍,需要新的治疗策略.
- 生物信息学确定了miR-5192和亡/TNF通路基因 (TNF,ZBP1,HSPA1B,MAPK3) 是主要的目标.
- ZBiotic是一种B. subtilis修饰的益生菌菌株,在NAFP中研究了其治疗潜力.
研究的目的:
- 在大鼠模型中评估ZBiotic对NAFP的治疗效果.
- 调查ZBiotic对涉及亡和TNF信号传递的特定基因的影响.
- 为了评估ZBiotic调节上游表观遗传调节者的能力,miR-5192.2.
主要方法:
- 鼠被食高脂肪,高糖 (HFHS) 饮食以诱导NAFP.
- 在NAFP模型大鼠中,每天服用ZBiotic (0.5和1毫升/公斤) 或原始B. subtilis (1毫升/公斤) 四周.
- 分析了基因表达,纤维化,炎症,胰岛素抵抗和胰腺细胞标记物.
主要成果:
- ZBiotic显著降低了miR-5192及其目标mRNA的调节 (p < 0.001).
- 治疗逆转了纤维化,炎症和胰岛素抵抗,降低了体重,血清粉酶和脂酶 (p < 0.001).
- 高剂量的ZBiotic显示出比原始菌株更高的疗效,降低了卡斯巴酶和NF-κB阳性细胞 (p <0.01).
结论:
- 在动物模型中,ZBiotic有效地减轻了NAFP的进展.
- 益生菌可以缓解纤维化,炎症和与NAFP相关的代谢异常.
- 治疗效果与亡/TNF介导途径的调节有关.
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