在强力下,FimH-曼诺斯非共价键能够在数分钟到数小时的时间内存活
Laura A Carlucci1, Keith C Johnson1, Wendy E Thomas1
1Department of Bioengineering, University of Washington, Seattle, Washington.
Biophysical journal
|July 4, 2024
概括
粘附FimH在尿路感染中至关重要,显示了与强力的结合寿命的增加. 这项研究测量了在强力下激活的FimH-曼诺斯键寿命,揭示了长期存在的抗强相互作用.
科学领域:
- 生物物理学的生物物理.
- 微生物学 微生物学
- 结构生物学 结构生物学
背景情况:
- 来自大肠杆菌的粘附FimH调解了细菌对宿主细胞的粘附.
- FimH表现出捕获债券行为,其中债券寿命随着施加力的增加而增加.
- 激活状态在强力下的寿命对于理解FimH功能至关重要,但仍然未测量.
研究的目的:
- 在不同的力量下测量激活的FimH-曼诺斯键的寿命.
- 为了研究FimH抗力粘附的结构基础.
- 通过测量激活依赖预负载力来确认FimH捕捉键行为.
主要方法:
- 多重复合磁子被用来应用预加载力并激活FimH-曼诺斯键.
- 激活的FimH-曼诺斯键的解离动力学被测量在一系列的力量.
- 酵母曼南被用作FimH.的基对象.
主要成果:
- 在临界预加载力以上,FimH债券的更高比例被激活,这证实了捕获债券的行为.
- 激活的FimH通过多态动力学从曼诺脱离,表明至少有两个不同的结合状态.
- 激活的FimH-曼诺斯键的平均寿命在30-70 pN的力量下从1000秒到10,000秒不等.
结论:
- 激活的FimH-曼诺斯键具有非常长的寿命和高抗力.
- 多态脱离动力学表明,在FimH-曼诺斯相互作用中存在复杂的结构重组.
- 这些发现提供了对抗力生物分子相互作用的结构性见解,与FimH介导的感染有关.
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