相关实验视频

Updated: Jul 6, 2026

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity

Published on: January 5, 2016

改变的S-AdenosylMethionine可用性影响了Saccharomyces cerevisiae中的dNTP池

Warunya Panmanee¹, Men T H Tran¹, Serigne N Seye¹

¹Department of Biological Sciences, Northern Kentucky University, Highland Heights, Kentucky, USA.

Yeast (Chichester, England)

|July 4, 2024

概括

在Saccharomyces cerevisiae SAM2基因中的突变降低了S-AdenosylMethionine (AdoMet) 水平,增加了基因组的不稳定性. 这种不稳定性与sam2∆/sam2∆细胞中除dTTP外的脱氧核酸三酸盐 (dNTP) 水平升高有关.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
生物化学生物化学

背景情况:

麦芽是一种用于研究基因组不稳定的模型生物.
AdoMet合成酶 (SAM1,SAM2) 产生S-AdenosylMethionine (AdoMet),这对于甲基化至关重要,并且与叶酸和甲基循环有关.
之前的研究表明,SAM1和SAM2删除不同影响AdoMet水平和基因组不稳定性.

研究的目的:

研究sam2∆/sam2∆酵母细胞中AdoMet水平,dNTP池和基因组不稳定性之间的关系.
确定AdoMet新陈代谢的改变如何影响脱氧核酸三酸盐 (dNTP) 的合成和稳定性.

主要方法:

在野生型和sam2∆/sam2∆ Saccharomyces cerevisiae菌株中对dNTP水平的比较分析.
对基因组不稳定性标记的评估与观察到的dNTP池变化相关.

主要成果:

与野生型相比,sam2∆/sam2∆细胞的AdoMet水平较低,基因组不稳定性增加.
这些细胞表现出高水平的dATP,dCTP和dGTP,为更大的dNTP总体池做出了贡献.
在sam2∆/sam2∆细胞中,dTTP水平没有显著改变.

结论:

由于AdoMet代谢发生变化,sam2∆/sam2∆细胞中的dNTP池增加可能导致基因组不稳定性增加.
无法控制的dNTP水平可能导致DNA损伤,这解释了这些酵母突变体中观察到的不稳定性.

关键词:

在AdoMet合成酶中.这种植物是Saccharomyces cerevisiae.S-adenosylmethionine (AdoMet) 是一种氨基基甲胺 (甲胺) 的一种.基因组的不稳定性甲基循环中的甲基循环.酵母遗传学酵母遗传学

更多相关视频

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

Published on: October 8, 2018

相关概念视频

Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase

Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase

Phase II biotransformation reactions are essential for detoxifying and eliminating xenobiotics, including many pharmaceutical compounds. These reactions typically involve conjugation, the covalent attachment of polar endogenous groups such as glucuronic acid, sulfate, methyl, or acetyl moieties to functional groups introduced during Phase I metabolism. The resulting conjugates are more water-soluble, enabling efficient renal or biliary excretion.The major classes of Phase II enzymes include...

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...

Biosynthesis of Nucleic Acids

Biosynthesis of Nucleic Acids

Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...