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Updated: May 5, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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一个基于微流体芯片的数字PCR精确量化基因编辑产品的平台

Jingzheng Chi1,2, Lin Ding1,2, Xiaofu Wang2

  • 1College of Advanced Agricultural Sciences, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.

Analytical methods : advancing methods and applications
|July 4, 2024
PubMed
概括

这项研究引入了一种基于微流体芯片的新型数字PCR平台,用于精确量化基因编辑产品. 该技术具有高灵敏度和低检测极限,可以准确评估各种作物的基因编辑效率.

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Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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