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根结线虫效应器MiEFF12针对宿主ER质量控制系统,以抑制免疫反应并允许寄生虫

Salomé Soulé¹, Kaiwei Huang², Karine Mulet¹

¹INRAE-Université Côte d'Azur-CNRS, UMR Institut Sophia Agrobiotech, Sophia Antipolis, France.

Molecular plant pathology

|July 4, 2024

概括

根结线虫 (RKN) 分泌12号效应因子 (EFF12),以操纵植物免疫力. 这种效应器向参与内质网质量控制的植物蛋白,帮助线虫寄生和作物损失.

科学领域:

植物病理学植物病理学
遗体学遗体学是一个学科.
分子植物-微生物相互作用

背景情况:

根结线虫 (RKN) 通过寄生于植物根部,导致全球作物损失显著.
螺旋虫操纵宿主植物细胞变成巨细胞以食,逃避植物的免疫力.
分泌的效应蛋白是关键的毒性因子,使线虫寄生成为可能.

研究的目的:

描述一个保存的RKN特异效应因子,效应因子12 (EFF12),并阐明其在寄生症中的作用.
在植物细胞内识别MiEFF12的宿主点.
调查EFF12对RKN毒性有所贡献的机制.

主要方法:

用RNA测序和免疫抑制生物测试来评估MiEFF12对宿主免疫力的影响.
酵母二杂交,分裂化酶和共免疫沉试验以确定蛋白质相互作用.
在 *Nicotiana benthamiana* 中进行基因沉默,以评估目标基因在易感性中的作用.

主要成果:

MiEFF12在线虫食道腺体中合成,并定位到植物内质网膜 (ER).
MiEFF12与Solanum lycopersicum bap-like (PBL) 和基本素拉链60 (BZIP60) 蛋白质相互作用,这些蛋白质是ER质量控制系统的组成部分.
在 *N. benthamiana* 中抑制 PBL 基因,降低了对 *Meloidogyne incognita* 感染的易感性.

结论:

EFF12是一个关键的RKN效应器,可以操纵植物免疫力.
MiEFF12的目标是ER质量控制组件 (PBL和BZIP60) 以促进寄生.
准PBL功能是RKN使用的一种策略,用于调节宿主防御并促进感染.

关键词:

梅洛伊多基尼隐形的身份.尼科蒂亚纳 (Nicotiana benthamiana) 是一种植物.这种植物是Solanum lycopersicum.质量控制ER质量控制ER质量控制效应器效应器是一个有效的效应器.细胞内膜网膜 (ER) 是一个

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