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选择性壁细胞招募细胞细胞到组装内皮细胞层管的网络中

Ksenia Yrigoin¹, George E Davis¹

¹Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida School of Medicine, Tampa, FL, United States.

Frontiers in cell and developmental biology

|July 4, 2024

概括

细胞,关键的壁画细胞,积极入侵3D矩阵并支持内皮管的发育. 血管光滑肌细胞在这些血管维护功能方面表现出有限的能力.

科学领域:

血管生物学血管生物学
细胞生物学细胞生物学
组织工程是组织工程.

背景情况:

壁细胞,包括细胞周细胞和血管光滑肌细胞 (VSMC),对于血管发育和维护至关重要.
细胞存在于毛细血管和静脉中,而VSMC存在于动脉和静脉等较大的血管中.

研究的目的:

为了研究和比较人体皮质细胞和人体冠状动脉光滑肌细胞 (CASMCs) 之间的功能差异.
评估它们在3D原基质入侵,3D原基质入侵,3D原基质入侵,3D原基质入侵,3D原基质入侵,3D原基质入侵,3D原基质入侵,3D原基质入侵,3D原基质入侵.

主要方法:

在3D原基质中与人类内皮细胞 (ECs) 共同培养人类细胞或CASMCs.
分析壁细胞入侵,招募到EC管,地下膜沉积的情况.
使用EC衍生因子的刺激,如血小板衍生生长因子 (PDGF) -BB,PDGF-DD和内甲蛋白-1.

主要成果:

与CASMCs不同的是,Pericytes在响应EC衍生的因素时显示出对3D原基质的显著入侵.
细胞有效地被招募到EC管网中,并在窄长的管道周围诱导地下膜沉积.
CASMCs显示出最小的招募和地下膜沉积,形成更宽,更短的管道.

结论:

与CASMC相比,细胞具有较高的功能能力,用于矩阵入侵,招募EC管,以及地下膜诱导.
这些发现突显出皮质细胞和VSMC在血管发育和维护中的独特作用.

关键词:

底层膜矩阵沉积沉积的地下膜.毛细血管是毛细血管的组成部分.它们是内皮细胞的内皮细胞.壁画的细胞是细胞.周围细胞 (pericytes) 是一个细胞.血管光滑肌肉细胞血管光滑肌肉细胞

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