卵白水解酸作为的抗微生物和抗炎药物
Anuwat Wanthong1,2, Chanapat Boonmark2, Nichakamol Vaisopha3
1Department of Biology, Faculty of Science, Mahasarakham University, Mahasarakham, 44150, Thailand.
Heliyon
|July 4, 2024
概括
研究人员使用性水解从蛋白中开发出抗菌. 这些酸具有较低的细胞毒性,并有效抑制像Cutibacterium acnes和MRSA这样的细菌,为感染治疗提供了潜力.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 材料科学 材料科学 材料科学
背景情况:
- 抗生素耐药性是一个日益严重的全球健康问题.
- 需要新的抗菌剂来对抗抗性细菌菌株.
- 像蛋白这样的自然来源为类发现提供了潜在的潜力.
研究的目的:
- 开发一种简单的方法,从蛋白中产生抗菌.
- 评估这些对相关细菌病原体的疗效.
- 研究作用机制和潜在的治疗应用.
主要方法:
- 性水解蛋白以产生.
- 在RAW 264.7巨细胞上进行细胞毒性测定.
- 细菌生长抑制测定对抗Cutibacterium acnes,金黄色葡萄球菌和MRSA.
- 氧化生产试验.氧化生产试验.
- 组学分析用于识别和表征.
- 电子显微镜可视化细菌细胞膜破坏.
主要成果:
- 一种可重复的方法产生了具有低细胞毒性的<14.4kDa.
- 卵白水解剂 (EWH) 抑制了C. acnes,S. aureus和MRSA的生长.
- 在C. acnes治疗的巨中,EWH减少了氧化的产生.
- 三个单独的 (PK8,EE9,RP8) 显示出抗菌和抗炎活性,但EWH更强.
- 作用机制涉及细菌细胞膜的破坏.
结论:
- 蛋白水解剂 (EWH) 和衍生具有显著的抗菌和抗炎性质.
- EWH是治疗感染的有希望的候选者,包括和MRSA.
- 这些发现支持开发用于治疗目的的天然抗菌剂.
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