以益生菌为基础的ORS补充剂适应热量可以通过改善肠道耐热性和调节大鼠肠道微生物群来缓解热中风引起的多器官功能障碍
Lei Li1,2,3, Juelin Chen1, Yawei Wang1
1Department of Emergency, Changhai Hospital, Naval Medical University, Shanghai, China.
Frontiers in microbiology
|July 4, 2024
概括
用益生菌为基础的口服补水盐 (ORSP) 进行热适应 (HA) 可以防止热中风 (HS). 这种组合疗法改善了热调节,减少了器官损伤,并增强了大鼠的肠道屏障功能和微生物群.
科学领域:
- 生理学 生理学 生理学
- 微生物学 微生物学
- 胃肠病学 胃肠病学
背景情况:
- 热中风 (HS) 是一种危及生命的疾病,死亡率高.
- 热适应 (HA) 是对HS的主要预防措施.
- 口服补水盐 (ORS) 和益生菌可以在HS期间支持肠道功能.
研究的目的:
- 在大鼠模型中研究HA与基于益生菌的ORS (ORSP) 结合对HS的影响.
- 评估ORSP辅助HA对温度调节,器官损伤,肠道屏障完整性和肠道微生物群的影响.
主要方法:
- 建立了一个大鼠HA模型,在21天内给予ORSP.
- 评估了热调节反应,器官损伤标志物,热冲击蛋白 (HSP) 表达和肠紧结 (TJ) 蛋白.
- 使用16S rRNA基因测序分析了肠道脂肪酸结合蛋白 (I-FABP),D-乳酸和肠道微生物群组成.
主要成果:
- 带有ORSP的HA减弱了HS诱导的高温症,并减少了多器官损伤.
- 通过HA,HSP90,HSP70,HSP60和HSP40的表达得到了上调.
- 有ORSP的HA降低了I-FABP和D-乳酸盐水平,保存了TJ蛋白 (ZO-1,E-cadherin,JAM-1),并改善了肠道屏障的完整性.
- 有益的细菌 (乳酸菌) 增加,而有害的细菌 (菌根菌) 减少.
结论:
- 与ORSP结合的HA增强了肠道的耐热性,并防止HS引起的损伤.
- 这一策略改善了肠道屏障功能,并有利地调节了肠道微生物群的组成.
- 由RSP协助的HA为预防和减轻热中风影响提供了一个有希望的方法.
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