储存储备和auxin的生物合成是由玉米内的层次性调节网络协调的
Teng Song1, Qiang Huo1, Chaobin Li1,2
1State Key Laboratory of Maize Bio-breeding, Frontiers Science Center for Molecular Design Breeding, Joint International Research Laboratory of Crop Molecular Breeding, National Maize Improvement Center, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
The New phytologist
|July 4, 2024
概括
转录因子ZmNAC128/130通过管理储存储量和通过层次网络调节印醇-3-酸 (IAA) 生物合成来协调玉米粒的填充. 这项研究揭示了新的目标和协调的监管机制.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 生物化学 生物化学
背景情况:
- 玉米 (Zea mays) 谷物填充取决于细胞发育,储存储备合成 (粉,蛋白质,脂质) 和植物激素调节.
- 控制玉米内精中的这些过程的精确监管网络尚未完全理解.
研究的目的:
- 阐明监管网络控制玉米谷物填充.
- 识别由ZmNAC128和ZmNAC130.0.调节的目标基因和下游转录因子 (TF).
- 研究ZmNAC128/130在储存储备和植物激素的生物合成中的作用.
主要方法:
- 染色体免疫沉测序 (ChIP-seq) 与RNA-seq相结合,用于识别ZmNAC128/130.0.的直接向基因.
- DNA亲和力净化测序,以构建下游TFs的层次性监管网络.
- 在玉米突变品中分析脂质和英多尔-3-酸 (IAA) 含量.
主要成果:
- 鉴定了1744个由ZmNAC128/130.0共同调节的高可信度目标基因.
- 发现了参与脂质和IAA生物合成的新型基因,除了粉和粉合成.
- 在zmnac128/130突变体中观察到油体,三糖醇和IAA的显著减少.
- 揭示了ZmNAC128/130及其下游TF之间的目标基因的显著重叠,特别是储存储备和IAA生物合成.
结论:
- ZmNAC128/130在协调玉米粒填充方面发挥着至关重要的作用.
- 一个多转录因子层次的监管网络控制了 storage reserves 和 IAA 在玉米内的生物合成.
- 这个网络整合了储存化合物和植物激素通路的调节,以实现最佳的谷物发育.
关键词:
监管网络是一个层次化的监管网络.印醇-3-乙酸的使用方法玉米的内精子储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储存储备储备储存储备储备储存储备储备储备储备储备储备储存储备储备储存储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储存储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储存储备储备储备储备储备储存储备储备储备储备储备储存储备储备储备储备储备储备储备储备储备储存储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储备储存储存储存储存储存储存储存储存储存储存储存储存储存储存储存储存储存储存储存储存转录因子是一种转录因子.相关概念视频
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