相关实验视频

Updated: Jun 22, 2025

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Published on: August 5, 2021

脂质滴滴动态对于疟疾寄生虫Plasmodium falciparum的发展至关重要

Jiwon Lee^1,2, Kai Matuschewski³, Giel van Dooren²

¹Centre for Advanced Microscopy, The Australian National University, Canberra ACT, 2601, Australia.

Journal of cell science

|July 4, 2024

概括

在感染期间,Plasmodium falciparum寄生虫中的脂质滴缩小,表明从脂质储存到利用的转变. 针对这些脂质滴提供了一种潜在的新策略来对抗疟疾.

科学领域:

寄生虫学的寄生虫学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

脂质滴 (LDs) 对于真核生物中的脂质和能量平衡至关重要.
疟疾寄生虫Plasmodium falciparum中LDs的功能尚不清楚,特别是关于脂质的获取和代谢用于膜合成.

研究的目的:

系统地描述Plasmodium falciparum血液阶段感染期间LDs的大小,组成和动态.
调查LDs在寄生虫分裂症和脂质平衡中的功能性作用.

主要方法:

分裂光发射分析. 分裂光发射分析.
三维 (3D) 聚焦离子束扫描电子显微镜 (FIB-SEM).
化学抑制三糖醇 (TAG) 的合成和分解.

主要成果:

在较晚的精神分裂阶段,LDs的尺寸会减少,这表明在寄生虫退出之前,人们会转向脂质利用.
确定了LDs与其他寄生虫有机体之间的联系,表明了潜在的功能相互作用.
抑制TAG合成或分解揭示了LDs在精神分裂症和管理脂质毒性的重要作用.

结论:

杆菌的LDs动态调节脂质的合成,储存和利用.
观察到的LD动态和功能是新型抗疟疾干预措施的潜在目标.

关键词:

原菌 (Plasmodium falciparum) 是一种有毒的病毒.3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D FIB-SEM 3D 先进的显微镜技术显微镜技术内红细胞阶段内红细胞期脂质滴滴是什么脂质滴滴是什么脂质新陈代谢脂质新陈代谢

更多相关视频

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum

Published on: December 4, 2015

A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting

A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting

Published on: May 16, 2013

相关概念视频

Symbiosis

Symbiosis

Symbiotic relationships are long-term, close interactions between individuals of different species that affect the distribution and abundance of those species. When a relationship is beneficial to both species, this is called mutualism. When the relationship is beneficial to one species but neither beneficial nor harmful to the other species, this is called commensalism. When one organism is harmed to benefit another, the relationship is known as parasitism. These types of relationships often...

Asymmetric Lipid Bilayer

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...

Fluid Mosaic Model

Fluid Mosaic Model

Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...