区域选择性氧化因子合由一个不特定的过氧酶
Lukas Platz1, Nikolai A Löhr2, Max P Girkens1
1Institute of Pharmaceutical Sciences, Albert-Ludwigs-Universität Freiburg, Albertstrasse 25, 79104, Freiburg, Germany.
Angewandte Chemie (International ed. in English)
|July 4, 2024
概括
研究人员在真菌中发现了一种新型酶,即非特异性过氧酶 (UPO),它负责催化氧化联合 (OPC) 以产生生物活性二元性素. 这一发现扩大了我们对不同真菌系的OPC生物合成的理解.
科学领域:
- 生物化学和分子生物学
- 菌类学 菌类学是指菌类学.
- 自然产品生物合成 自然产品生物合成
背景情况:
- 双 (前) antraquinones 是通过氧化联合 (OPC) 合成的广泛存在的天然产品.
- 像P450s,过氧酶和乳酶这样的酶催化OPC,但形成的真菌 (Basidiomycota) 中的酶仍然没有表征.
- 在菌 (Ascomycota) 中OPC的生物合成是众所周知的,这凸显了对其他真菌群体的知识差距.
研究的目的:
- 为了阐明素A1和B1的生物合成,从Cortinarius odorifer.的亚特罗皮索默.
- 为了识别和描述在Basidiomycota中负责OPC的酶.
- 扩大已知的氧化联反应的酶谱.
主要方法:
- 在Aspergillus niger.中异构复合的黄素A1和B1生物合成.
- 涉及的O-甲基转移酶 (CoOMT1) 和非特异性过氧酶 (CoUPO1) 的识别和功能性表征.
- 分析由CoUPO1.1催化的区域选择性同性结合.
主要成果:
- 该研究确定了CoOMT1,它负责甲基化酸到酸.
- 发现CoUPO1,一种非特异性过氧酶,可催化torosachrysone的区域选择性同类合,以产生phlegmacins.
- 这代表了对非特异性过氧酶的前所未有的反应,扩大了已知的OPC的生物催化作用范围.
结论:
- 贝西迪奥米科塔利用非特异性过氧酶进行选择性合,独立于其他生物体.
- 这一发现揭示了OPC的新型酶途径,扩大了对天然产品生物合成的理解.
- 氧化烯合在不同的真菌系中发挥着核心和多样化的作用.
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