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Updated: Jun 22, 2025

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

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从螺旋式中间体中制备的α-synuclein多态分子的固态NMR赋值

Sahil Ahlawat1, Surabhi Mehra2, Chandrakala M Gowda3

  • 1Tata Institute of Fundamental Research, Sy. No. 36/P, Gopanpally Village, Serilingampally Mandal, Ranga Reddy District, Hyderabad, 500 046, India. sahilr@tifrh.res.in.

Biomolecular NMR assignments
|July 4, 2024
PubMed
概括

合成核蛋白病变涉及阿尔法-合成核蛋白蛋白聚合物. 这项研究揭示了不同蛋白质中间体产生的明显的α-synuclein纤维结构 (多态),可能解释各种疾病病理.

关键词:
任务分配 任务分配纤维素是纤维的组成部分.寡合体是一种寡合体.固态NMR是一种固态NMR.α 合成核蛋白.

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In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is...
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The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
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At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
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