通过FTIR显微光谱仪对感染急性炎症组织进行无标签的检查,使用无监督和监督的分析方法
Jingzhu Shao1, Xiangyu Zhao1, Ping Tang1
1Center for Biophotonics, Institute of Medical Robotics, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
概括
福里埃变换红外光谱 (FTIR) 显微镜有效地区分了急性肌炎 (AP) 脏组织与健康组织. 这种无标签的方法分析了分子和形态变化,在分类受感染的组织中实现了高精度.
科学领域:
- 生物医学光学 生物医学光学
- 频谱学是一种光谱学.
- 病理学 病理学 病理学
背景情况:
- 急性肺炎 (AP) 是一种严重的尿路感染 (UTI),影响全球许多患者.
- 目前AP的诊断方法包括尿液分析,成像和组织学.
- 福里埃变换红外光谱 (FTIR) 显微镜为分析组织形态和分子组成提供了一种无标签的方法.
研究的目的:
- 研究FTIR显微光谱在AP的老鼠模型中分析脏组织的实用性.
- 使用FTIR光谱数据,区分正常的脏组织 (皮质和髓) 和感染的AP组织.
- 评估基于FTIR光谱的无监督和监督机器学习方法的准确性,用于根据FTIR光谱对脏组织进行分类.
主要方法:
- 使用FTIR显微镜来收集来自老鼠脏组织的光谱数据.
- 使用无监督方法 (集成,聚类,PCA) 来创建红外组织学图.
- 使用支持矢量机 (SVM) 进行监督分析,用于组织分类.
- 基于同步的FTIR被用于特定脏结构的高分辨率成像.
主要成果:
- 与H&E染色相比,红外组织图成功区分了脏组织类型.
- 分析显示,受感染的AP组织中的二胺二酸减少,核酸和脂质增加.
- PCA有效地将感染者与正常组织分开.
- 在SVM模型中,用于区分组织类型的分类精度达到96.11%.
- 使用同步射线FTIR生成了高分辨率的质和死细胞质量图.
结论:
- FTIR显微镜是研究AP组织的强大工具.
- 这种技术可以在模型系统中将受感染的组织与正常组织区分开来.
- FTIR为AP诊断提供了一个无标签的,分子信息化的方法.
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