低蛋白S表达诱导了软骨中的氧化损伤
Yixin Cui1, Yucheng Liao1, Yonghui Chen1
1School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, No. 76 Yanta West Road, Xi'an, Shaanxi 710061, People's Republic of China.
概括
低 (Se) 水平通过增加氧化应激会损害软骨健康. 减少Selenoprotein S (Selenos) 表达导致细胞损伤和软骨生长受损,突出显示Selenos.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 微量元素的新陈代谢
背景情况:
- (Se) 是一种重要的微量元素,对生物功能至关重要.
- 蛋白S (Selenos) 参与细胞平衡和抗氧化剂防御.
- 氧化应激会破坏细胞功能,并导致各种病理.
研究的目的:
- 为了研究低水平的SELENOS基因表达对红细胞中氧化应激的影响.
- 确定SELENOS在维持软骨抗氧化能力方面的作用.
- 阐明将SELENOS缺乏与软骨功能障碍联系在一起的机制.
主要方法:
- 使用SELENOS-knockdownATDC5细胞系来评估氧化应激标记.
- 使用SELENOS敲击C57BL/6小鼠来评估对软骨的体内影响.
- 测量了二乙,活性氧物种,马隆迪阿尔代海德,8-基-2-脱氧氨酸和超氧化物溶解酶 (SOD) 表达的水平.
- 分析了核因子红色素2相关因子2/血红素酶-1信号通路.
主要成果:
- 治疗SELENOS的细胞显示氧化应激标志物 (二乙,活性氧物种,) 的增加和SOD表达的减少.
- 在SELENOS的淘汰赛中,小鼠的8-基-2-脱氧瓜诺辛水平升高,软骨中SOD的表达减少.
- 低水平的SELENOS表达与增加的核因子红色素2相关因子2/血红素酶-1信号相关,有助于氧化损伤.
- 缺少SELENOS导致软骨生长板中的生殖区缩短,表明分化受损.
结论:
- 低水平的Selenos表达显著促进了软骨中的氧化应激.
- 豆在维护软骨的抗氧化能力和结构完整性方面发挥着至关重要的作用.
- 对SELENOS的失调可能导致软骨分化功能障碍,并可能导致与软骨相关的疾病.
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