原蛋白通过调节内分泌网膜应激应变来改善的合成
Liling Wu1, Jiayuan Qu2, Li Mou2
1NHC Key Laboratory of Birth Defects and Reproductive Health, Chongqing Population and Family Planning Science and Technology Research Institute, Chongqing 401120, China; Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, China; Department of Pharmacy, Nanchong Central Hospital, The Second Clinical School of North Sichuan Medical College, Nanchong, Sichuan 637000, China; Nanchong Key Laboratory of Individualized Drug Therapy, Nanchong, Sichuan 637000, China.
原蛋白 (AP) 可以帮助减少男性肥胖和改善丸功能,通过减轻内分泌网膜压力 (ERS) 和恢复莱迪格细胞中的合成.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 代谢疾病 代谢疾病
背景情况:
- 男人肥胖是一个与不孕症相关的日益蔓延的流行病.
- 肥胖会破坏荷尔蒙平衡,特别是水平.
- 天然产品在治疗代谢疾病方面表现有前途.
研究的目的:
- 为了研究阿皮金因在抵消高脂肪饮食引起的丸功能障碍方面的潜力.
- 探索涉及细胞内网膜应激 (ERS) 和合成的机制.
- 评估阿皮基宁对肥胖和脂质代谢的影响.
主要方法:
- 使用高脂肪饮食 (HFD) 建立了一种肥胖的小鼠模型.
- 服用阿皮基宁 (10毫克/公斤) 并评估生理,组织学和分子变化.
- 使用在实验室研究的小鼠莱迪格细胞 (TM3) 与自由脂肪酸 (FFAs).
- 采用ChIP,qPCR和基因沉默来分析基因表达和蛋白质相互作用.
主要成果:
- 在肥胖小鼠中,阿皮基宁 (AP) 管理降低了体重,改善了脂质配置文件.
- AP改善了丸病理,并恢复了与丸相关的蛋白质水平.
- 在体外,AP缓解了ERS,并在莱迪格细胞中恢复了的合成.
- 通过CHOP调制,保护AP免受ESR诱导的类固醇性STAR表达的抑制.
结论:
- 原蛋白在缓解高脂肪饮食引起的肥胖和男性丸功能障碍方面表现出显著的潜力.
- 原蛋白的保护作用与减轻内分泌网膜应激和恢复合成有关.
- 向ESR和增强激素生产是阿皮基宁治疗作用的关键机制.
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