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整个转录组分析确定了与丝虫,Bombyx mori的男性不育相关的差异表达的mRNA,miRNA和lncRNA

Tianchen Huang¹, Shanshan Zhong¹, Juan Sun¹

¹College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China; Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Scientific Research Center, Chinese Academy of Agricultural Sciences, Zhenjiang 212100, China.

Comparative biochemistry and physiology. Part D, Genomics & proteomics

|July 4, 2024

概括

这项研究使用转录组分析研究了丝虫的男性不育性. 研究结果揭示了参与氨基酸生物合成和蛋白质合成的关键基因通路,为昆虫无菌技术开发提供了洞察力.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
昆虫学昆虫学是一门学科.

背景情况:

昆虫无菌技术对于虫虫害控制至关重要.
识别男性不育基因是推动这项技术发展的关键.
丝虫 (JMS) 突变为研究男性不孕症提供了一个模型.

研究的目的:

为了阐明JMS丝虫突变体中男性不育的形成机制.
在雄性无菌丝中识别差异表达基因 (DElncRNAs,mRNAs,DEmiRNAs).
探索男性不孕不育背后的监管网络.

主要方法:

对JMS和野生型丝虫丸的全转录组分析.
识别和量化差异表达的非编码RNA,信使RNA和微RNA.
对差异表达基因的KEGG通路丰富分析.
竞争的内源性RNA (ceRNA) 调控网络的构建和分析.

主要成果:

在JMS和野生型丝虫之间确定了205个DElncRNA,913个mRNA和92个DEmiRNA.
凯格分析显示了氨基酸生物合成和ECM受体相互作用途径的丰富.
ceRNA网络分析表明,氨基酸生物合成和蛋白质合成途径在男性不孕症中起着作用.

结论:

这项研究深入了解了丝虫中男性不育基因的调节网络.
在JMS突变体中确定了对男性不孕不育至关重要的特定基因通路.
为开发先进的昆虫不育技术提供了一个新的视角和潜在的目标.

关键词:

氨基酸的生物合成波姆比克斯 (Bombyx mori mori mori) 是一个有趣的动物.对于ECM与受体的相互作用.男人的不孕不育能力.整个转录组的转录组.ceRNARNA是什么意思

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