相关实验视频

Updated: Jun 22, 2025

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Published on: June 10, 2020

对ADAR基因补充,表达模式和Chlamys farreri中的RNA编辑景观的洞察

Enrico Bortoletto¹, Umberto Rosani¹, Akari Sakaguchi²

¹Department of Biology, University of Padova, 35121, Padova, Italy.

Fish & shellfish immunology

|July 4, 2024

概括

海洋贝拥有六种对RNA (ADARs) 基因起作用的腺氨酸脱氨酶,对抗病毒防御至关重要. 它们的表达和编辑活动在发育过程中增加,这表明它们在幼虫阶段对抗病毒感染的适应性作用.

科学领域:

分子生物学分子生物学
海洋生物学海洋生物学
基因组学就是基因组学.

背景情况:

作用于RNA的腺氨酸脱氨酶 (ADARs) 是参与RNA编辑和宿主病毒相互作用的重要酶.
克拉米斯·法雷里海容易感染急性病毒性亡病毒 (AVNV),导致显著的死亡率.

研究的目的:

在Chlamys farreri中识别和描述ADAR转录和基因.
为了研究ADAR基因表达模式和RNA编辑活动在贝发育期间和在不同的组织.
评估ADARs在贝抗病毒防御中的潜在作用.

主要方法:

转录组数据的元分析.
对ADAR基因的全基因组识别和注释.
遗传学分析. 遗传学分析.
使用RNA-seq. 的基因表达造型.
对ADAR介导的RNA编辑部位的分析.

主要成果:

在Chlamys farreri中发现了8个ADAR转录和6个ADAR基因,包括新的替代拼接变体.
遗传学分析将C. farreri的5个ADAR置于ADAR1类中.
基因表达分析揭示了差异性的表达模式,CF051320在成年组织中高度表达,并在早期发育过程中增加.
通过ADAR介导的RNA编辑主要检测到跨组织和发育阶段的跨基因区域.
通过ADAR1调解的编辑显示,在幼虫定居前阶段,活动增加.

结论:

在Chlamys farreri中发现的六个ADAR基因具有独特的功能域,并表现出不同的表达特征.
发育和组织特异性的表达模式,以及编辑活动,表明ADAR1介导的RNA编辑在贝抗病毒免疫力中的适应性作用,特别是在生命早期.

关键词:

这是ADAR编辑.双免疫力双免疫力发展发展发展发展发展伊诺西尼 (Inosine) 是一种蛋白质.

更多相关视频

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

Published on: January 30, 2020

Chromatin Immunoprecipitation ChIP using Drosophila tissue

Chromatin Immunoprecipitation ChIP using Drosophila tissue

Published on: March 23, 2012

相关概念视频

RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

Position-effect Variegation

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...

Lampbrush Chromosomes

Lampbrush Chromosomes

In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...

Chromatin Position Affects Gene Expression

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...

Cis-regulatory Sequences

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...