抗生素耐药性基因在典型的医院废水处理过程中,与宿主致病性和移动性相关的风险存在
Chenye Xu1, Chun Hu2, Fang Li1
1College of Environmental Science and Engineering, Donghua University, Shanghai, 201620, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, China.
Environmental research
|July 4, 2024
概括
医院废水处理减少了抗生素耐药性基因 (ARG),但消毒增加了它们. 这项研究强调了移动ARG和致病细菌带来的风险,强调了需要更好的干预措施的必要性.
科学领域:
- 环境微生物学 环境微生物学
- 废水处理 废水处理
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 医院废水是抗生素耐药基因 (ARG) 和抗生素耐药细菌 (ARB) 的重要来源.
- 在HWW治疗期间,ARG的动态,致病性,流动性和抗药性风险尚未得到充分理解.
研究的目的:
- 在HWW治疗过程中调查ARG,致病性ARB和移动遗传元素 (MGE) 的发生和转移.
- 评估整体抗生素耐药性风险指数 (ARRI) 和处理过程的影响,特别是消毒.
主要方法:
- 甲基因组测序和组装用于在18个HWW样本中识别ARG亚型和宿主细菌.
- 基于连接的分析确定了与ARG相关的致病性ARB和MGE.
- 抗生素耐药性风险指数 (ARRI) 计算以评估整体风险.
主要成果:
- 确定了817种ARG亚型,其中多药物,氨基糖化物和β-乳酸盐耐药性基因最为普遍.
- 废水处理将ARG降低了77.4%,直到二次沉积,但消毒增加了它们在废水中的丰度.
- 蛋白质细菌宿主15种ARG类型;发现了114种致病性ARB,包括多药耐药的大肠杆菌和 Pseudomonas物种.
- 7.10%-31.0%的ARG与MGEs有关,转体酶 (tnpA) 显示出高传播潜力,通常具有β-乳糖抗性基因.
结论:
- 原始影响物构成最高的抗体风险,其次是废水,消毒通过促进致病性ARB和移动ARG而加剧了风险.
- 了解ARG载体,水平基因转移和治疗影响对于管理临床环境和接受环境中的耐药性风险至关重要.
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