通过Alum:CpG复杂辅助剂促进COVID-19疫苗中的免疫反应
Jia Ji1, Taoming Tang1, Miaojin Zhu1
1State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, China.
/CpG辅助剂显著提高了小鼠对非活化COVID-19疫苗的免疫反应,增加了抗体标位和T细胞激活. 这种组合辅助剂增强了对三角洲变种的保护,表明长期免疫力的潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 病毒学 病毒学
背景情况:
- 辅助剂对疫苗有效性至关重要,但对它们对COVID-19无活化疫苗的影响的数据有限.
- 了解辅助体特异性免疫反应对于开发有效的SARS-CoV-2疫苗至关重要.
研究的目的:
- 评估不同辅助剂 (Alum,CpG HP021,Alum/CpG,MF-59) 与非活化COVID-19疫苗的疗效.
- 为了比较K18-hACE2和BALB/c小鼠模型中的免疫反应.
- 为了评估对SARS-CoV-2Delta变种的保护有效性,挑战.
主要方法:
- 无活化COVID-19疫苗的配方包括,CpG HP021,/CpG或MF-59辅助剂.
- 在K18-hACE2和BALB/c小鼠中分析了免疫反应,包括抗体标位,同型,细胞因子生产和T细胞种群.
- 用SARS-CoV-2德尔塔变种挑战小鼠,以评估疫苗的保护.
主要成果:
- /CpG辅助剂显著增加了抗RBDIgG抗体标位和抗体产生,即使抗原减少.
- /CpG和CpG HP021促进了Th1/IgG2c反应,而和MF-59则支持Th2/IgG1.
- /CpG疫苗接种增强了T细胞反应 (IFN-γ,IL-2,效能记忆CD8+T细胞) 并保护K18-hACE2小鼠免受Delta变异挑战.
结论:
- /CpG复合剂显著增强了对非活化COVID-19疫苗的免疫反应.
- 这种辅助组合引起了强大的细胞和幽默免疫力,包括中和抗体和T细胞反应.
- /CpG辅助剂在开发有效和潜在的长期无活化COVID-19疫苗方面显示出前景.
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