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在非洲多队列研究中,症状性结核病的血液转录学签名
Vanessa Mwebaza Muwanga1, Simon C Mendelsohn1, Vinzeigh Leukes2
1South African Tuberculosis Vaccine Initiative, Institute of Infectious Disease and Molecular Medicine, Division of Immunology, Department of Pathology, University of Cape Town, Cape Town, South Africa.
评估了20个血液转录组签名,作为结核病 (TB) 的非唾液选试验. 虽然在聚合分析中没有一个符合世界卫生组织目标产品概况标准,但一些在特定国家表现有前途.
科学领域:
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
- 诊断 诊断 诊断 诊断
背景情况:
- 结核病 (TB) 诊断通常依赖于液,需要使用其他非液方法.
- 主体血液的转录特征正在被探索为潜在的结核病分类测试.
研究的目的:
- 为了比较20个血液转录组签名的诊断性能.
- 在有症状的患者中区分结核病 (TB) 和其他呼吸道疾病 (ORD).
主要方法:
- 一项嵌套病例控制研究招募了来自六个非洲国家的初级医疗保健诊所的有症状的个体.
- 结核病诊断通过临床,微生物学和放射学评估得到证实.
- 使用微流体实时定量PCR测量了全血转录基因特征.
主要成果:
- 9个签名在区分结核病和ORD方面表现相似 (AUC从0.79到0.83).
- 特异性在90%的灵敏度下从44%到54%不等,不符合世界卫生组织目标产品 (TPP) 标准.
- 签名表现因艾滋病毒状况和国家而异;一些签名在埃塞俄比亚,马拉维和南非等特定国家符合TPP标准.
结论:
- 在综合分析中,在所有评估国家中,没有一个单一的血液转录组签名符合非吐结核病分拣测试的TPP标准.
- 然而,某些签名显示出在特定国家背景下作为分拣测试的潜力,需要进一步调查.
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