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在人类血液和血中多重检测和识别病毒,细菌和原生动物病原体,使用扩展高密度再测序微阵列平台

Moussa Kourout¹, Scott Espich¹, Carolyn Fisher¹

¹Division of Emerging and Transfusion Transmitted Diseases, Office of Blood Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, United States.

Frontiers in molecular biosciences

|July 5, 2024

概括

多重测试平台,如血液传递病原体复序微阵列扩展 (BBP-RMAv.2) 提供高效的病原体检测血液安全. 这项研究表明,BBP-RMAv.2在血液样本中有效识别病毒,细菌和寄生虫.

科学领域:

分子生物学分子生物学
传染病诊断传染病诊断传染病诊断
公共卫生公共卫生

背景情况:

核酸检测提高了血液供应的安全性,但新兴病原体查具有挑战性.
多重测试平台为简化病原体检测提供了可行的解决方案.

研究的目的:

评估血液传染病原体复序微阵列扩展 (BBP-RMAv.2) 用于在人类血液或血中检测多重病原体.
为了比较BBP-RMAv.2微阵列平台与纳米孔下一代测序 (NGS) 的性能.

主要方法:

利用BBP-RMAv.2平台对80种病毒,细菌和寄生虫进行多重检测.
测试的人类血液/血样本中添加了精选的病毒,细菌和原生动物病原体.
提取核酸,用多重聚合原料放大它们,并将其混合为微阵列用于病原体识别.
将微阵列结果与纳米孔下一代测序 (NGS) 进行比较,以直接比较性能.

主要成果:

在BBP-RMAv.2检测到病毒病原体的度低至100拷贝/毫升.
该平台准确地识别了多种度范围 (1,000-100,000副本/毫升) 的尖端病原体.
纳米孔测序显示出高精度,识别了大多数样本,并在识别病原体负样本方面表现优于BBP-RMAv.2.

结论:

BBP-RMAv.2平台在血液安全和疾病诊断方面显示出多重病原体检测的巨大潜力.
无论是BBP-RMAv.2还是NGS都是有效的分子方法来识别血液中的病原体.
与BBP-RMAv.2微阵列相比,NGS在区分病原体负样本方面表现出更好的表现.

关键词:

通过血液传播的病原体.微阵列的微阵列多重检测检测多重检测再测序微阵列的使用方法有针对性的测序.

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