RNA-seq分析揭示了转录组重编程和替代拼接在早反应盐应激在番茄根的早期反应
Jianghuang Gan1,2, Yongqi Qiu1,2, Yilin Tao1,2
1Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou, Zhejiang, China.
Frontiers in plant science
|July 5, 2024
概括
盐应激显著影响番茄根基因表达和替代拼接 (AS). 这项研究揭示了增强的AS事件,特别是外跳跃,为开发耐盐作物提供了洞察力.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 压力生理学 压力生理学
背景情况:
- 盐应激严重限制了农作物的生产力,因为它损害了植物的生长.
- 了解分子对盐度的反应对于开发耐盐作物至关重要.
- 替代拼接 (AS) 调节基因表达和蛋白质组多样性,影响植物对非生物压力的反应.
研究的目的:
- 分析转录组变化并描述早期盐应激反应期间番茄根的替代拼接 (AS) 景观.
- 为了确定关键的基因和转录因子参与盐应激适应.
- 为改善番茄盐耐受性提供一种资源.
主要方法:
- 使用高通量RNA测序,在盐应力下分析番茄根转录组.
- 进行了差异基因表达 (DEG) 和差异替代拼接 (DAS) 分析.
- 识别参与拼接机械的转录因子和基因.
主要成果:
- 超过10,588个基因被差异地表达,显着参与激素信号传递,氨基酸代谢和细胞循环调节.
- 超过700个转录因子 (TF) 被确定为盐反应的潜在调节者.
- 替代拼接 (AS) 显著增强,外因子跳转是最常见的事件,影响了3709个基因.
结论:
- 盐应激强烈刺激番茄根中的替代拼接 (AS).
- 编码血清/三联蛋白激酶,含有重复 (PPR) 蛋白质和E3无素蛋白连接酶的基因显著地被替代地拼接.
- 超过100个与拼接机械相关的差异表达基因 (DEG) 表明在盐反应性AS事件中起着调节作用.
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