相关实验视频
Updated: Jun 22, 2025
13:10
Use of Alu Element Containing Minigenes to Analyze Circular RNAs
Published on: March 10, 2020
7.3K
小RNA介导的遗传开关协调ALG-3/4小RNA路径的功能
Trilotma Sen1, Cara McCormick1, Alicia K Rogers1
1Department of Biology, University of Texas at Arlington, Arlington, TX 76019, USA.
Nucleic acids research
|July 5, 2024
概括
这项研究揭示了一种新的RNA干扰 (RNAi) 到RNAi调节级联在C. elegans. 这种机制通过在发育和热应激期间暂时控制阿尔戈纳特的表达来确保精子的生育能力和耐热性.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 发展生物学 发展生物学
背景情况:
- 基因调节网络需要精确的协调细胞功能.
- RNA干扰 (RNAi) 是甲基动物中保存的基因调节机制.
- 在整个发育阶段和环境变化中保持RNAi稳态是至关重要的.
研究的目的:
- 研究控制C. elegans中阿尔戈诺亚特ALG-3和ALG-4表达的调控机制.
- 阐明小干扰RNAs (siRNAs) 在调节这些阿尔戈纳特人中的作用.
- 了解这种调节级联如何促进精子生育能力和耐热性.
主要方法:
- 对alg-3和alg-4基因的siRNA调节的分析.
- 研究ALG-3和ALG-4的时间表达模式.
- 评估这种调节级联在热应激下对精子生育能力的影响.
主要成果:
- 阿尔戈纳特人ALG-3和ALG-4的表达是由siRNAs调节的.
- 一个由siRNAs操作的时间基因开关机制控制阿尔戈纳特人的表达.
- 这种RNAi-to-RNAi级联对于协调ALG-3/4通路功能至关重要,确保耐热生育.
结论:
- 一个新的RNAi-to-RNAi调节级联在发育和压力期间保持RNAi稳态.
- 这种机制通过对阿尔戈纳特人的时间控制来确保精子的生育能力和耐热性.
- 在其他物种中保存的RNAi通路可能采用类似的调节架构.
相关概念视频
01:23RNA Interference
26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
02:15Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
01:56Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
01:23Types of RNA
63.5K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.5K
02:30siRNA - Small Interfering RNAs
16.7K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.7K
02:18Alternative RNA Splicing
21.1K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.1K