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链杆菌 spp. 的二次代谢物概况. 当与银纳米颗粒的次致命度生长时的变化:可能对新型化合物发现的潜在影响

Merajuddin Khan¹, Hamad Z Alkhathlan¹, Syed Farooq Adil¹

¹Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, 11451, Riyadh, Saudi Arabia.

Antonie van Leeuwenhoek

|July 5, 2024

概括

亚致命的银纳米粒子 (Ag-NPs) 诱导了土壤细菌中的抗菌化合物产生. 这种新的策略增强了对抗耐药病原体的新化合物的发现.

科学领域:

微生物学微生物学
纳米技术纳米技术
药物发现药物发现药物发现

背景情况:

多抗药性病原体的兴起和新抗生素的减少管道需要针对抗菌素化合物发现的创新方法.
动态细菌是一种丰富的生物活性天然产品来源,但许多生物合成基因集群保持沉默或神秘.

研究的目的:

调查亚致命银纳米颗粒 (Ag-NPs) 的潜力,以诱导来自Actinobacteria的新生物活性二次代谢物的产生.
为了确定特定的Streptomyces菌株及其代谢物,在暴露于Ag-NPs时负责抗菌活性.

主要方法:

42个沙特土壤Actinobacteria分离物被培养成具有或没有亚致命的Ag-NP (50μg/ml).
消耗的汁被选为抗菌活性对黄金葡萄球菌和白杆菌.
使用16S rRNA基因测序识别了活性菌株. 使用气体染色学-质谱学 (GC-MS) 和液体染色学-质谱学 (LC-MS) 分析了二次代谢物概况.

主要成果:

在42个菌株中,有3个菌株在与Ag-NPs一起生长时,对S. aureus和M. luteus表现出抗菌活性.
两种菌株,分别称为Streptomyces labedae和Streptomyces tirandamycinicus,被选择进行进一步研究.
GC-MS和LC-MS分析显示,在存在Ag-NP的情况下,二次代谢物概况发生了显著的变化,但醇提取物对M. luteus和S. aureus具有强烈活性.

结论:

亚致死度的Ag-NPs有效调节Streptomyces物种中的二次代谢物产生.
这种Ag-NP介导的诱导策略显示出揭示神秘的生物合成途径和发现新的抗菌化合物的前景.
已识别的代谢物可能与氧化应激反应有关,并具有抗微生物特性,为对抗耐药细菌提供了新的途径.

关键词:

新型抗微生物药物新型抗微生物药物在PKS基因中,PKS基因是基因.二次代谢产物二次代谢产物银纳米颗粒银纳米粒子这种病名为Streptomyces.

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