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相关概念视频

Upstream Processing01:27

Upstream Processing

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Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
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Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells MSCs and Endothelial Colony Forming Progenitor Cells ECFCs
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从不同的人类组织来源中分离的MSC的上游处理协议.

Pelin Kılıç1,2, Cansu Özdemir3,4, Begüm Coşar5,6

  • 1Department of Stem Cells and Regenerative Medicine, Stem Cell Institute, Ankara University, Ankara, Türkiye. pkilic@ankara.edu.tr.

Methods in molecular biology (Clifton, N.J.)
|July 5, 2024
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概括

介酶体 stromal/干细胞 (MSC) 生产对于再生医学至关重要. 本研究详细介绍了MSC生产的优化实验室规模协议,支持先进治疗药物 (ATMP).

关键词:
先进疗法药物 (ATMPs) 是一种先进疗法药物.骨髓衍生中酶体干细胞/干细胞 (BM-MSCs)细胞培养培养的细胞培养.结冷保存是一种冷保存.良好的制造实践 (GMP) 是一个隔离 隔离 隔离 隔离主细胞银行 (MCB)经过的旅行 经过的旅行生产周围组织衍生中细胞/干细胞 (PNT-MSCs)工作细胞银行 (WCB)

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科学领域:

  • 细胞生物学 细胞生物学
  • 再生医学是一种再生医学.
  • 生物技术是生物技术.

背景情况:

  • 介酶体 stromal/干细胞 (MSCs) 在再生医学和细胞疗法中越来越重要.
  • 人们越来越感兴趣于MSCs用于各种医疗应用,包括先进治疗药物 (ATMP).
  • 细胞培养技术的进步使MSC在良好制造实践 (GMP) 下的大规模生产成为可能,确保了安全性和有效性.

研究的目的:

  • 介绍MSC生产日益重要的意义.
  • 概述对MSCs在各种医疗应用中的日益增长的兴趣.
  • 描述一个优化的上游协议,用于实验室规模的MSC生产.

主要方法:

  • 为实验室规模的MSC生产开发一个优化的上游协议.
  • 使用不同的组织来源进行MSC隔离.
  • 在控制关键参数的瓶中进行细胞培养.
  • 为ATMP生成下游处理奠定了基础.

主要成果:

  • 描述了MSC生产的优化实验室规模协议.
  • 该协议控制了关键参数,以实现一致的MSC生成.
  • 描述的方法为下游处理提供了基础,以产生ATMP.

结论:

  • 在再生医学中,MSC生产具有显著的临床应用潜力.
  • 量身定制的生产流程对于产生安全有效的ATMP至关重要.
  • 优化的协议促进了实验室规模的MSC生产,支持未来的治疗开发.