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Updated: Jun 21, 2025

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

对Xenopus自组织信号通路的分子分析,从蛋到尾茎的轴向模式

Yagmur Azbazdar¹, Edward M De Robertis¹

¹Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA 90095-1662.

Proceedings of the National Academy of Sciences of the United States of America

|July 5, 2024

概括

鸟胚胎的发育依赖于顺序信号传递. 关键发现揭示了Chordin和Nodal可以诱导β-catenin耗尽胚胎的完整轴,独立于β-catenin活动,突出显示新的发育途径.

科学领域:

发展生物学发展生物学
细胞信号传递细胞信号传递
胚胎发生是胚胎发生.

背景情况:

背腹 (D-V) 和前后 (A-P) 轴形成对于胚胎发育至关重要.
了解控制Xenopus胚胎细胞分化的顺序信号通路是必不可少的.

研究的目的:

在Xenopus胚胎中剖析控制D-V和AP细胞分化的信号通路.
研究特定信号分子和对手在轴形成中的作用.

主要方法:

在Xenopus胚胎中使用了功能丧失和功能获取方法.
微注射特定的mRNA和蛋白质 (Hwa,β-catenin,节点相关因子,BMP4,Dkk1).
使用β-catenin激活Luciferase记者和表型救援实验分析信号通路活动.

主要成果:

华蛋白激活早期β-catenin信号,诱导双胞胎轴.
与Xenopus节点相关的因素拯救β-catenin耗尽,并被Cerberus-Short和Lefty抑制.
BMP抗体Chordin和Noggin在没有β-catenin的胚胎中拯救D-V和AP组织,令人惊的是没有β-catenin的转录活性.
迪克科夫 (Dkk1) 通过抑制晚期Wnt信号与Hwa协同作用;它的效果由Sizzled (Szl) 调节.
BMP4抑制了Hwa诱导的轴,而Dkk1则抑制了晚期的BMP信号传递.

结论:

胚胎轴形成涉及复杂的,上下文依赖的信号相互作用.
在特定条件下,Chordin和Nodal可以独立于β-catenin转录活性来诱导完整的胚胎轴.
迪克科普f1的前部化和背部化作用是由晚期BMP信号调节的介导,需要Sizzled.
化诱导的脊柱化依赖于节点.

关键词:

科尔贝鲁斯 (Cerberus) 是一个类动物.合唱团合唱团合唱团合唱团迪克的头部有11个胡鲁瓦瓦 (Huluwa) 是一个古老的城市.这是一种β-catenin.

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