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可扩展的脂质滴滴微阵列的制造,验证和选.

Tracey N Bell1, Aubrey E Kusi-Appiah1, Vincent Tocci1

  • 1Department of Biological Science and Integrative NanoScience Institute, Florida State University, Tallahassee, Florida, United States of America.

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概括

脂滴微阵列通过向细胞输送脂友化合物,使得可扩展,小型化药物查成为可能. 该方法可适应各种细胞类型,适用于空间有限或细胞有限的设置.

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科学领域:

  • 生物技术是生物技术.
  • 药物发现 药物发现 药物发现
  • 细胞生物学 细胞生物学

背景情况:

  • 高通量选是必不可少的,但成本高昂,受到空间和试剂要求的限制.
  • 微阵列对一些测试是有效的,但由于脂性化合物输送挑战,它没有被广泛采用用于基于细胞的药物发现.
  • 脂质滴滴微阵列提供了一种解决方案,以微型格式将脂性药物送到细胞中.

研究的目的:

  • 扩大脂质滴滴微阵列制造,试验验证和药物选过程.
  • 为适应纳米印以进行可扩展的阵列生产,并为水性环境稳定阵列.
  • 为了证明脂质滴滴微阵列在向各种细胞类型输送脂性和水溶性化合物的实用性.

主要方法:

  • 采用印刷机进行适应的纳米印,用于可扩展的微型阵列制造.
  • 使用蒸汽涂层工艺稳定脂质滴滴阵列,用于浸泡水溶液.
  • 用细胞外矩阵蛋白质功能化阵列,并证明将化合物传递给三种细胞类型.

主要成果:

  • 成功扩大了脂质滴滴阵列制造和稳定技术.
  • 证明成功地将脂性和水溶性化合物输送到细胞中.
  • 在0.1厘米2的微阵列上选了92种天然产品提取物,显示了广泛的适用性.

结论:

  • 脂滴微阵列为小型化基于细胞的药物查提供了一个可扩展和多功能平台.
  • 这项技术适用于高生物安全水平的设施,空间有限的环境和细胞数量有限的精密医学应用.