相关实验视频

Updated: Jun 21, 2025

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
10:50

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

16.4K

一个细胞竞争系统,其中一个基因表达来自一个单复制基因的单个细胞中的一个基因

Yoshinori Hasegawa1, Megumi Nakano2, Tsutomu Hosouchi1

  • 1Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.

PloS one
|July 5, 2024
PubMed
概括

一个新的转基因竞争系统使单细胞中的多个基因能够同时表达. 这种方法准确地比较了基因对细胞增殖的影响,揭示了核受体之间的显著差异.

更多相关视频

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria06:08

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

Published on: January 25, 2019

12.9K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.7K

相关概念视频

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.3K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

4.6K
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.3K
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
167.3K
Complementary DNA01:44

Complementary DNA

Overview
29.4K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K