通过调节膜胆固醇载体,LncRNA DANCR促进了巨细胞脂质的积累

相关实验视频

Updated: Jun 21, 2025

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

Guo-Jun Zhao¹, Yu Wang¹, Jun-Hong An²

¹Affiliated Qingyuan Hospital, Guangzhou Medical University (Qingyuan People’s Hospital), Qingyuan 511518, Guangdong, China.

|July 5, 2024

概括

长非编码RNA DANCR促进巨细胞中的脂质积累,导致动脉样硬化. 抑制DANCR可以减少脂质滴并增强胆固醇流量,为冠状动脉疾病提供潜在的治疗点.

科学领域:

心血管生物学心血管生物学
分子医学是分子医学.
动脉样硬化研究动脉样硬化研究

背景情况:

动脉样硬化 (AS),冠状动脉疾病 (CAD) 的一个关键因素,涉及大量的脂质沉积在血管壁.
长非编码RNAs (lncRNAs) 与脂质疾病和AS有关,但lncRNA DANCR在动脉生成中的特定作用尚不清楚.

研究的目的:

研究lncRNA DANCR在巨细胞脂质积累中的作用及其在动脉样硬化背景下的潜在机制.
确定DANCR是否影响膜胆固醇运输蛋白的表达,并影响巨细胞中的胆固醇稳态.

主要方法:

定量PCR (qPCR) 用于测量CAD患者和动脉样硬化小鼠的DANCR水平.
在体外研究中,使用用ox-LDL治疗的THP-1和RAW264.7巨细胞来评估DANCR表达和脂质积累.
油红色O染色测量脂质滴滴;西部斑点和胆固醇流量测试评估胆固醇运输蛋白 (ABCA1,ABCG1,SR-BI,CD36,SR-A).
涉及DANCR抑制 (siRNA) 和过度表达的实验,以确定对脂质吸收和排泄的功能影响.

主要成果:

在CAD患者,动脉样硬化小鼠和用牛LDL治疗的巨细胞中,DANCR表达显著更高.
DANCR 抑制减少了脂质滴积累,而 DANCR 过度表达增加了巨细胞中的脂质滴积累.
DANCR调节了胆固醇运输蛋白 (ABCA1,ABCG1,SR-BI) 和胆固醇排放的表达.
DANCR通过调节清理器受体 (CD36,SR-A) 来影响氧化低密度脂蛋白 (ox-LDL) 的吸收.
观察到的DANCR的效应与miR-33a.独立.

结论:

lncRNA DANCR通过促进巨细胞脂质积累,在泡细胞形成中起着有害的作用.
DANCR通过其对胆固醇运输蛋白和LDL吸收的影响来调节巨细胞脂质代谢.
DANCR代表了预防或治疗动脉样硬化的潜在治疗标.

关键词:

在丹麦,丹克尔.胆固醇的运输胆固醇的运输脂质的积累脂质的积累巨细胞是一个巨细胞.这就是miR-33a.

更多相关视频

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Published on: April 3, 2017

Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death

Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death

Published on: October 31, 2017

相关概念视频

Inflammation

Inflammation

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis

Cholesterol: Significance and Regulation

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...