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SlWRKY37针对SlLEA2和SLABI5-like7来调节西红中的种子发芽活力

Zhirong Wang¹, Jialong Zhang², Ming Gao³

¹College of Horticulture, China Agricultural University, Beijing, 100193, China; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Plant physiology and biochemistry : PPB

|July 5, 2024

概括

研究人员发现,番茄中的SlWRKY37基因在种子休眠中起着关键作用. 淘汰这种基因可以改善种子发芽,为旨在减少休眠期的育种计划提供了潜力.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
农业科学农业科学

背景情况:

种子发芽是植物生命周期的一个重要阶段,受遗传和环境因素的影响.
种子休眠是一个复杂的特征,可以限制作物建立和产量.

研究的目的:

研究SlWRKY37转录因子在调节番茄 (Solanum lycopersicum) 种子发芽和休眠期中的功能.
确定SlWRKY37在种子生理学中的作用背后的分子机制.

主要方法:

使用CRISPR/Cas9基因编辑创建SlWRKY37淘汰赛和过度表达线.
在突变系上进行了RNA测序 (RNA-Seq) 和基因本体学 (GO) 丰富分析.
生物信息学和生物化学测试被用来识别直接的转录标和酸化位.

主要成果:

在番茄种子发芽期间,SlWRKY37的表达减少.
SlWRKY37淘汰突变体表现出增强的发芽,而过度表达线则显示出延迟的发芽.
突变物中差异表达的基因与种子休眠,酸 (ABA) 稳态和蛋白质酸化有关.
确定了SlaBI5-like7和Slalea2是SlWRKY37的直接目标,它们调节了种子休眠状态.
在Ser65中对SlWRKY37的酸化对其转录活性至关重要.

结论:

SlWRKY37作为西红种子发芽和休眠期的负调节剂.
了解SlWRKY37的功能为种子休眠调节提供了洞察力.
SlWRKY37是基因操纵的潜在目标,以改善番茄育种中的种子发芽特征.

关键词:

酸化是指酸化的方法.种子休眠状态种子休眠状态在SLABI5-like7中使用了SLABI5.这种植物是Solanum lycopersicum.转录式激活方式

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