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双反应单链聚合物纳米颗粒通过活性和点击化学进行直角功能化

Jan-Willem D Paats¹, Naomi M Hamelmann¹, Jos M J Paulusse¹

¹Department of Molecules and Materials, MESA+ Institute for Nanotechnology and TechMed Institute for Health and Biomedical Technologies, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500, AE, Enschede, the Netherlands.

Journal of controlled release : official journal of the Controlled Release Society

|July 5, 2024

概括

研究人员通过结合PFP-和点击化学开发了双重功能纳米粒子. 用葡萄糖装饰的纳米颗粒显示癌细胞的细胞吸收增加,突出了创造多功能纳米载体的多功能策略.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
聚合物化学聚合物化学

背景情况:

葡萄糖是生物医学应用中纳米粒子的关键向配体.
单链聚合物纳米粒子 (SCNP) 是多功能5-20纳米纳米载体.
SCNP的表面功能化对于有针对性的交付至关重要,但目前的方法有局限性.

研究的目的:

开发双反应单链聚合物纳米粒子 (SCNP),结合PFP-和点击化学.
为了创建具有不同葡萄糖密度和附着位置的葡萄糖功能化SCNP (glyco-SCNP).
为了评估这些糖-SCNP在HeLa癌细胞中的细胞吸收.

主要方法:

合成的SCNP与可变的五二 (PFP) .
通过PFP化学与1-amino-3-butyne功能化的SCNP产生butyne-SCNP.
使用铜(I) 催化酸基环添加 (CuAAC) 将合的3-酸葡萄糖转化为布丁-SCNP. 点击化学.
在HeLa癌细胞中评估细胞吸收.

主要成果:

通过整合PFP-和CuAAC点击化学,成功创建了双反应性SCNP.
生成的葡萄糖-SCNP具有可调的表面葡萄糖密度和不同的葡萄糖附着位置 (C1或C6).
观察到糖-SCNP的细胞吸收增加,具有更高的葡萄糖表面密度,独立于附着位置.

结论:

开发的双结合策略为SCNP表面功能化提供了增强的多功能性.
用葡萄糖装饰的SCNP显示出向癌细胞传递的潜力.
这种方法可以为各种生物医学应用创建多功能SCNP的多种库.

关键词:

活性 Ester 是一种活性.点击"化学"就可以了.控制的激素聚合.葡萄糖是一种葡萄糖.单链聚合物纳米粒子单链聚合物纳米粒子表面功能化的功能化.添加了醇-迈克尔的添加物.

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