储存在EDTA管中的血液提供了48小时内准确的花生基激活测试结果
Anthony A Horner1, Michael H Land2, Rania Abolhosn1
1Department of Immune Diagnostics, Quest Diagnostics Nichols Institute, San Juan Capistrano, California.
概括
使用EDTA血液的花生基激活测试 (BAT) 稳定两天,保持高诊断准确度. 这一发现支持其在基准实验室中用于诊断花生过敏的临床应用.
科学领域:
- 过敏和免疫学 过敏和免疫学
- 诊断测试 诊断测试 诊断测试
- 临床实验室科学 临床实验室科学
背景情况:
- 花生基激活测试 (BAT) 显示了高的诊断准确性与肝素化血液.
- 临床实用性受到肝素化血液中短时间样本稳定性的限制.
研究的目的:
- 为了确定花生BAT样品的稳定性是否可以使用EDTA抗凝血血液延长到2天.
- 为了满足美国参考实验室的最低稳定性要求.
主要方法:
- 招募的儿童 (6个月-17岁) 有不同的花生敏感性.
- 收集EDTA抗凝血血液样本,运送到中央实验室,并在4°C下存储1-2天.
- 在112名儿童的双盲研究中验证了标准.
主要成果:
- 84-86%的花生BAT结果在储存1-2天后可以解释.
- 实现了高诊断准确率95.5% (第一天) 和94.4% (第二天).
- 在检测花生过敏时表现出100%的灵敏度.
结论:
- 在收集后2天内,EDTA抗凝血血液样本在花生BAT测试中稳定.
- 花生BAT表现出良好的诊断性能,可用于临床使用.
- 支持在商业参考实验室中使用花生BAT进行花生过敏诊断.
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